Wang He, Li Zhang-Chun, Luo Zhen-Hua, Chen Zheng-Hong
Department of Microbiology, Guiyang Medical College, Guiyang, Guizhou 550004, China.
Zhonghua Nan Ke Xue. 2008 Jul;14(7):583-9.
To establish the rat model of chronic bacterial prostatitis and investigate the penetrability of amikacin to chronic inflammatory prostatic tissues.
A total of 180 male rats were randomly divided into a normal control group (NC, n=48), a chronic bacterial prostatitis group (CBP, n = 84) and a CBP treatment group (CBPT, n = 48). The prostates of the animals were injected with Xiaozhiling and E. coli respectively to make CBP and CBPT models. The animals of the CBPT group were treated with amikacin by intramuscular injection, their prostate tissues and sera isolated at 1-150 min after the treatment and detected for antibiotic activities, bacteria counts and pathological changes.
Obvious chronic inflammatory pathological changes including leukocyte invasion and fibre hyperplasia were observed and E. coli isolated from the prostate tissues of the rats in the CBP and CBPT groups, but no pathological changes, antibiotic activity and bacteria were detected in the the NC group. The numbers of E. coli in the prostate tissues markedly decreased with the time in the two model groups, 30 CFU/mg in the CBP rats at 28 days and 0 CFU/mg in the CBPT group at 10 days after the treatment. Obvious antibiotic activities were found in both the prostate tissues and sera at 2-150 min after the injection. No antimicrobial activities were detected at 12 hours after the treatment either in the sera or in the prostate samples. With the increase of the treatment time and decrease of the bacteria counts, the chronic inflammatory pathological changes were obviously alleviated in the CBPT group.
Rat models of CBP with chronic inflammatory pathological changes can be successfully established by direct injection of Xiaozhiling and E. coli into the prostate. Amikacin, given by intramuscular injection, can penetrate into the prostate of the CBP rat and produce an antibiotic activity equal to or higher than that of the sera, which may kill sensitive bacteria in the prostate and help to reduce the inflammatory pathological changes and repair the damage to the prostate tissues.
建立大鼠慢性细菌性前列腺炎模型，研究阿米卡星对慢性炎性前列腺组织的渗透能力。
将180只雄性大鼠随机分为正常对照组（NC，n = 48）、慢性细菌性前列腺炎组（CBP，n = 84）和CBP治疗组（CBPT，n = 48）。分别向动物前列腺内注射消痔灵和大肠杆菌以制作CBP和CBPT模型。CBPT组动物肌肉注射阿米卡星治疗，于治疗后1 - 150分钟采集其前列腺组织和血清，检测抗生素活性、细菌计数及病理变化。
CBP组和CBPT组大鼠前列腺组织出现明显的慢性炎性病理变化，包括白细胞浸润和纤维增生，且从前列腺组织中分离出大肠杆菌，而NC组未检测到病理变化、抗生素活性及细菌。两个模型组前列腺组织中的大肠杆菌数量随时间显著减少，治疗后28天CBP大鼠为30 CFU/mg，治疗后10天CBPT组为0 CFU/mg。注射后2 - 150分钟在前列腺组织和血清中均发现明显的抗生素活性。治疗后12小时血清和前列腺样本中均未检测到抗菌活性。随着治疗时间延长和细菌数量减少，CBPT组慢性炎性病理变化明显减轻。
通过向前列腺直接注射消痔灵和大肠杆菌可成功建立具有慢性炎性病理变化的CBP大鼠模型。肌肉注射阿米卡星可渗透至CBP大鼠前列腺并产生等于或高于血清的抗生素活性，这可能杀灭前列腺中的敏感细菌，有助于减轻炎性病理变化并修复前列腺组织损伤。
