Lee Yong Seok, Han Chang Hee, Kang Sung Hak, Lee Seung-Ju, Kim Sae Woong, Shin Ok Ran, Sim Young-Chul, Lee Sang-Jun, Cho Yong-Hyun
Department of Urology, College of Medicinem The Catholic University of Korea, Seoul, Korea.
Int J Urol. 2005 Apr;12(4):383-9. doi: 10.1111/j.1442-2042.2005.01052.x.
Chronic bacterial prostatitis (CBP) is the most common urological disease in adult men. Antibiotic therapy is the gold standard of treatment. However, long-term therapy resulted in many side-effects and bacterial resistance. Because of these reasons, we need new treatment modality that could replace traditional antibiotic therapy. Catechin, an extract of green tea, has antimicrobial effect against various bacteria and synergy effect to antibiotics. We evaluate the synergistic effects of catechin on the treatment of CBP in an animal model.
An experimental CBP model was induced in 70 male Wistar rats by instillation of 0.2 mL bacterial suspension (E-coli Z17, O2:K1:H-) containing 1 x 10(8) CFU/mL into the prostatic urethra. Microbiologically and histologically proven CBP model was demonstrated in 58.6% (41 of 70) of the rats after 4 weeks of bacterial instillation. The 41 rats demonstrating CBP were randomly divided into four groups; the control, catechin, ciprofloxacin and catechin with ciprofloxacin groups. All drug treatments were conducted over a period of 2 weeks. After treatment, the results were analyzed with microbiological cultures and histological findings of the prostate and urine samples to compare each group.
Microbiological cultures and histological findings of the prostate and urine samples demonstrated reduced bacterial growth and improved inflammatory responses in all three experimental groups compared with the control group. The catechin group showed coherent trends of decrease in bacterial growth and improvements in prostatic inflammation compared with the control group, but not to a statistically significant degree (P > 0.05). However, the ciprofloxacin and catechin with ciprofloxacin groups showed statistically significant decreases in bacterial growth and improvements in prostatic inflammation compared with the control group (P < 0.05). The catechin with ciprofloxacin group showed a statistically significant decrease in bacterial growth and improvements in prostatic inflammation compared with the ciprofloxacin group (P < 0.05).
These results suggest that catechin may be an effective material in CBP treatment. Particularly, combination treatment of catechin and ciprofloxacin has synergistic effect. Therefore, we suggest that the combination of catechin and ciprofloxacin may be effective in treating CBP with a higher success rate.
慢性细菌性前列腺炎（CBP）是成年男性中最常见的泌尿系统疾病。抗生素治疗是其治疗的金标准。然而，长期治疗会导致许多副作用和细菌耐药性。由于这些原因，我们需要能够替代传统抗生素治疗的新治疗方式。儿茶素是绿茶的提取物，对多种细菌具有抗菌作用，并与抗生素具有协同效应。我们在动物模型中评估儿茶素对CBP治疗的协同作用。
通过向前列腺尿道注入0.2 mL含1×10⁸CFU/mL的细菌悬液（大肠杆菌Z17，O2:K1:H-），在70只雄性Wistar大鼠中诱导建立实验性CBP模型。在注入细菌4周后，58.6%（70只中的41只）的大鼠经微生物学和组织学证实为CBP模型。将这41只表现为CBP的大鼠随机分为四组；对照组、儿茶素组、环丙沙星组和儿茶素与环丙沙星联合组。所有药物治疗持续2周。治疗后，通过对前列腺和尿液样本进行微生物培养及组织学检查来分析结果，以比较各组情况。
与对照组相比，前列腺和尿液样本的微生物培养及组织学检查显示，所有三个实验组的细菌生长均减少，炎症反应得到改善。与对照组相比，儿茶素组细菌生长呈下降趋势，前列腺炎症有所改善，但差异无统计学意义（P>0.05）。然而，与对照组相比，环丙沙星组和儿茶素与环丙沙星联合组细菌生长显著减少，前列腺炎症得到改善（P<0.05）。与环丙沙星组相比，儿茶素与环丙沙星联合组细菌生长显著减少，前列腺炎症得到改善（P<0.05）。
这些结果表明儿茶素可能是CBP治疗中的一种有效物质。特别是，儿茶素与环丙沙星联合治疗具有协同效应。因此，我们认为儿茶素与环丙沙星联合可能有效治疗CBP，成功率更高。
