Yang Ji-hong, Meng Xian-xian, Xie Li-sha, Guo Zheng
Shanxi Eye Hospital, Shanxi, People's Republic of China.
Neurosci Lett. 2008 Oct 10;443(3):218-22. doi: 10.1016/j.neulet.2008.07.073. Epub 2008 Aug 5.
Evidence indicates that sympathetic nerves and substance P (SP) are involved in some physiological and pathophysiological changes and activities in retina. The aim of this study was to investigate whether SP participates in the stress reaction and possible involvement of adrenergic mechanisms in modulation of the changes of SP in the retina of the rats suffering from acute stress evoked by coronary artery occlusion (CAO). The changes of SP in retina were examined and analyzed within 6h of CAO using immunohistochemistry, in situ hybridization and EIA approaches. The effects of phentolamine, an antagonist of alpha1-adrenergic receptor, and esmolol, an antagonist of beta1-adrenergic receptor, on the changes of SP were investigated to examine the role of adrenergic mechanisms in modulation of expression of SP in the retina under the stressful condition. It was observed that SP was markedly up-regulated in the layers of ganglion cells, inner plexiform, inner nuclear and pigment epithelium within 6h of the CAO. Intravenous administration of the adrenergic antagonists attenuated the up-regulation of SP. The results may indicate that SP in retina was involved in the stress response induced by acute myocardial ischemia. Adrenergic mechanisms may modulate the process in the retina.
有证据表明，交感神经和P物质（SP）参与视网膜的一些生理和病理生理变化及活动。本研究旨在探讨SP是否参与应激反应，以及肾上腺素能机制是否可能参与调节冠状动脉闭塞（CAO）诱发的急性应激大鼠视网膜中SP的变化。采用免疫组织化学、原位杂交和酶免疫分析方法，在CAO后6小时内检测和分析视网膜中SP的变化。研究α1肾上腺素能受体拮抗剂酚妥拉明和β1肾上腺素能受体拮抗剂艾司洛尔对SP变化的影响，以探讨应激状态下肾上腺素能机制在调节视网膜中SP表达的作用。观察到在CAO后6小时内，神经节细胞层、内网状层、内核层和色素上皮层中的SP明显上调。静脉注射肾上腺素能拮抗剂可减弱SP的上调。结果可能表明，视网膜中的SP参与了急性心肌缺血诱导的应激反应。肾上腺素能机制可能调节视网膜中的这一过程。
