Department of Comprehensive Examination for Eye Diseases, Shanxi Eye Hospital, Taiyuan, Shanxi, China.
Neurosci Lett. 2011 Jan 3;487(1):110-2. doi: 10.1016/j.neulet.2010.10.006. Epub 2010 Oct 16.
Evidence indicates that adaptive responses to systemic stress occur in retina, as indicated by the changes in sympathetic and sensory afferent neurotransmitters in the retina when under stressful stimulation, such as acute myocardial infarction. The aim of this study was to investigate the potential association of the adaptive responses with retina injury, indicated as apoptosis of retinal cells of the rats suffering from acute stress evoked by coronary artery occlusion (CAO). The apoptotic rate of retinal cells and activity of caspase-3 in retina were examined and analyzed over 6 h of CAO using TUNEL and caspase assay. It was observed that the rate of apoptosis of the retinal cells was significantly increased to 16.8%±6% (n=10, vs. control, p<0.05) and 26.9%±6.1% (n=10, vs. control, p<0.05), mainly in ganglion cells of the retina, at 3 and 6h of CAO, respectively. And the activity of caspase-3 was also up-regulated to 2.16±0.77 at 3h of CAO (vs. 1.00±0.32 of the control, p<0.05, n=10) and 2.72±1.11 at 6h of CAO (vs. 1.37±0.76 of control, p<0.05, n=10). The findings may indicate that the adaptive responses to acute stressful simulation, such as acute myocardial infarction, could result in retinal cell injury, presented as increase in apoptosis of retinal cells.
证据表明，全身应激会引起视网膜产生适应性反应，例如在急性心肌梗死等应激刺激下，视网膜中的交感神经和感觉传入神经递质会发生变化。本研究旨在探讨这种适应性反应与视网膜损伤之间的潜在关联，具体表现为急性应激引起的冠状动脉阻塞（CAO）后大鼠视网膜细胞的凋亡。通过 TUNEL 和半胱氨酸天冬氨酸蛋白酶（caspase）检测，分析了 CAO 后 6 小时内视网膜细胞的凋亡率和 caspase-3 的活性。结果发现，CAO 后 3 小时和 6 小时，视网膜细胞的凋亡率分别显著增加至 16.8%±6%（n=10，与对照组相比，p<0.05）和 26.9%±6.1%（n=10，与对照组相比，p<0.05），主要发生在视网膜神经节细胞中。同时，caspase-3 的活性也分别上调至 CAO 后 3 小时的 2.16±0.77（与对照组的 1.00±0.32 相比，p<0.05，n=10）和 CAO 后 6 小时的 2.72±1.11（与对照组的 1.37±0.76 相比，p<0.05，n=10）。这些发现表明，急性应激模拟（如急性心肌梗死）引起的适应性反应可能导致视网膜细胞损伤，表现为视网膜细胞凋亡增加。
