Seigle-Murandi F, Steiman R, Benoit-Guyod J L
Laboratoire de Botanique, Cryptogamie, Biologie Cellulaire, et Génétique, UFR de Pharmacie, Université Joseph Fourier (Grenoble I), Meylan, France.
Ecotoxicol Environ Saf. 1991 Jun;21(3):290-300. doi: 10.1016/0147-6513(91)90068-z.
A first screening was performed upon 100 strains of micromycetes cultivated on solid media (malt extract medium and mineral medium) with pentachlorophenol (PCP) (0.5 g/liter). Under these conditions, 50 strains gave a light blurring around the inoculation spot, indicating some PCP degradation. Later, 50 selected strains were cultivated in liquid synthetic medium with PCP (1 g/liter). After 6 days of cultivation, photodegradation occurred for 25%. On the whole, the consumption of PCP was 25% for Zygomycetes, 3% for yeasts, and 10-15% for Deuteromycetes, except 7% for Tuberculariales. It was shown that glucose repressed the PCP consumption. Among degrading fungi, some could grow with PCP when cultures were initiated with spores, others could not. A more detailed study was done with Phoma glomerata cultivated in liquid synthetic medium (PCP 100 mg/liter) in darkness or with light. Photodegradation increased up to 25% but abiotic degradation occurred also in darkness (8%). Consumption of PCP by Ph. glomerata was 27% after 2 days with light and was lower in darkness (19%).
对在含有五氯苯酚（PCP）（0.5克/升）的固体培养基（麦芽提取物培养基和矿物培养基）上培养的100株微真菌进行了首次筛选。在这些条件下，50株菌株在接种点周围出现轻微模糊，表明有一定程度的五氯苯酚降解。随后，将50株筛选出的菌株在含有PCP（1克/升）的液体合成培养基中培养。培养6天后，25%发生了光降解。总体而言，接合菌纲对PCP的消耗量为25%，酵母菌为3%，半知菌类为10 - 15%，除瘤座孢目为7%。结果表明，葡萄糖会抑制PCP的消耗。在降解真菌中，一些真菌在以孢子开始培养时能够利用PCP生长，而另一些则不能。对在黑暗或光照条件下于液体合成培养基（PCP 100毫克/升）中培养的聚生茎点霉进行了更详细的研究。光降解增加至25%，但在黑暗中也会发生非生物降解（8%）。光照2天后，聚生茎点霉对PCP的消耗量为27%，黑暗中消耗量较低（19%）。
