GDP-岩藻糖：蜜蜂（意大利蜜蜂）毒腺中β-N-乙酰葡糖胺（岩藻糖转移至（岩藻糖α1----6N-乙酰葡糖胺）-天冬酰胺-肽）α1----3-岩藻糖基转移酶活性。天冬酰胺结合的N-乙酰葡糖胺的双岩藻糖基化作用。
GDP-fucose: beta-N-acetylglucosamine (Fuc to (Fuc alpha 1----6GlcNAc)-Asn-peptide)alpha 1----3-fucosyltransferase activity in honeybee (Apis mellifica) venom glands. The difucosylation of asparagine-bound N-acetylglucosamine.
作者信息
Staudacher E, Altmann F, Glössl J, März L, Schachter H, Kamerling J P, Hård K, Vliegenthart J F
机构信息
Institut für Chemie, Universität für Bodenkultur, Vienna, Austria.
出版信息
Eur J Biochem. 1991 Aug 1;199(3):745-51. doi: 10.1111/j.1432-1033.1991.tb16179.x.
Incubation of honeybee (Apis mellifica) venom-gland extracts with GDP-[14C]fucose and GlcNAc beta 1----2Man alpha 1----6(GlcNAc beta 1----2Man alpha 1----3)Man beta 1----4GlcNAc beta 1----4(Fuc alpha 1----6)GlcNAc beta 1----N-Asn-peptide(NAc) gave a labeled product in 40% yield. Analysis by 500-MHz 1H-NMR spectroscopy indicated the transferred fucose-(Fuc) residue to be alpha 1----3-linked to the Asn-bound GlcNAc. Further proof was provided by one-dimensional and two-dimensional 1H-NMR analysis of the incubation mixture, after incubation with beta-N-acetylhexosaminidase. The established carbohydrate structure (formula; see text) proves the existence of a novel alpha 1----3-fucosyltransferase with the ability to effect difucosylation of the Asn-bound GlcNAc in N-glycans.
将蜜蜂（意大利蜜蜂）毒腺提取物与GDP-[14C]岩藻糖及GlcNAcβ1----2Manα1----6(GlcNAcβ1----2Manα1----3)Manβ1----4GlcNAcβ1----4(Fucα1----6)GlcNAcβ1----N-天冬酰胺-肽(NAc)一起温育，得到了产率为40%的标记产物。通过500兆赫的1H-NMR光谱分析表明，转移的岩藻糖（Fuc）残基以α1----3连接到与天冬酰胺结合的GlcNAc上。在用β-N-乙酰己糖胺酶温育后，对温育混合物进行一维和二维1H-NMR分析，提供了进一步的证据。所确定的碳水化合物结构（分子式；见正文）证明存在一种新型的α1----3-岩藻糖基转移酶，它能够使N-聚糖中天冬酰胺结合的GlcNAc发生双岩藻糖基化。
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