在椎实螺结缔组织中鉴定出一种GDP-岩藻糖：β1-3-半乳糖-N-乙酰半乳糖胺-R（岩藻糖到半乳糖）α1-2岩藻糖基转移酶和一种GDP-岩藻糖：β1-4-N-乙酰葡糖胺（岩藻糖到N-乙酰葡糖胺）α1-3岩藻糖基转移酶。
Identification of a GDP-Fuc:Gal beta 1-3GalNAc-R (Fuc to Gal) alpha 1-2 fucosyltransferase and a GDP-Fuc:Gal beta 1-4GlcNAc (Fuc to GlcNAc) alpha 1-3 fucosyltransferase in connective tissue of the snail Lymnaea stagnalis.
作者信息
Mulder H, Schachter H, Thomas J R, Halkes K M, Kamerling J P, Vliegenthart J F
机构信息
Bijvoet Center, Department of Bio-Organic Chemistry, Utrecht University, The Netherlands.
出版信息
Glycoconj J. 1996 Feb;13(1):107-13. doi: 10.1007/BF01049686.
Connective tissue of the freshwater pulmonate Lymnaea stagnalis was shown to contain fucosyltransferase activity capable of transferring fucose from GDP-Fuc in alpha 1 -2 linkage to terminal Gal of type 3 (Gal beta 1-3GalNAc) acceptors, and in alpha 1-3 linkage to GlcNAc ot type 2 (Gal beta 1-4GlcNAc) acceptors. The alpha 1-2 fucosyltransferase was active with Gal beta 1-3GalNAc beta 1-OCH2CH=CH2 (Km = 12mM, V(max) = 1.3 mUml-1) and Gal beta 1-3GalNAc (km =20 mM, V(max) = 2.1 mUml-1), whereas the alpha 1-3 fucosyltransferase was active with Gal beta 1-4GlcNAc (Km = 23 mM, V(max) = 1.1 mUml-1). The products formed from from Gal beta 1-3GalNAc beta 1-OCH2CH=CH2 and Gal beta 1-4GlcNAc were purified by high performance liquid chromatography, and identified by 500 MHz 1H-NMR spectroscopy and methylation analysis to be Fucalpha1-2Gal beta 1-3GalNAc beta 1-OCH2CH=CH2 and Gal beta 1-4(Fucalpha1-3)GlcNAc, respectively. Competition experiments suggest that the two fucosyltransferase activities are due to two distinct enzymes.
淡水肺螺类椎实螺的结缔组织被证明含有岩藻糖基转移酶活性，该酶能够将GDP - Fuc中的岩藻糖以α1 - 2连接方式转移到3型（Galβ1 - 3GalNAc）受体的末端Gal上，并以α1 - 3连接方式转移到2型（Galβ1 - 4GlcNAc）受体的GlcNAc上。α1 - 2岩藻糖基转移酶对Galβ1 - 3GalNAcβ1 - OCH2CH = CH2（Km = 12mM，V(max) = 1.3 mUml-1）和Galβ1 - 3GalNAc（km = 20 mM，V(max) = 2.1 mUml-1）具有活性，而α1 - 3岩藻糖基转移酶对Galβ1 - 4GlcNAc（Km = 23 mM，V(max) = 1.1 mUml-1）具有活性。由Galβ1 - 3GalNAcβ1 - OCH2CH = CH2和Galβ1 - 4GlcNAc形成的产物通过高效液相色谱法纯化，并通过500 MHz 1H - NMR光谱和甲基化分析鉴定，分别为Fucα1 - 2Galβ1 - 3GalNAcβ1 - OCH2CH = CH2和Galβ1 - 4（Fucα1 - 3）GlcNAc。竞争实验表明，这两种岩藻糖基转移酶活性是由两种不同的酶引起的。
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