Xu C B, Verwaerde C, Grzych J M, Fontaine J, Capron A
Centre d'Immunologie et Biologie Parasitaire, Unité Mixte INSERM U 167-CNRS 624, Institut Pasteur, Lille, France.
Eur J Immunol. 1991 Aug;21(8):1801-7. doi: 10.1002/eji.1830210804.
The protective effects of two different monoclonal antibodies (mAb) raised against the Schistosoma mansoni 28-kDa glutathione S-transferase (Sm 28 GST) were investigated. Two mAb of the same isotype (IgM) have been selected according to the blocking effect on Sm 28 GST enzymatic activity (S13) or the lack of blockade (H12). When passively transferred into Fischer rats, both S13 and H12 significantly reduced the worm burden. In BALB/c mice clear effects on female worm fecundity and egg viability were observed when the S13 mAb was transferred; these effects included significantly reduced loads of intestinal eggs, reduced egg hatching rates and an increased proportion of non-living eggs. No effect on egg production and egg hatching was observed in H12-treated mice. In addition, worm pairs recovered from S13-but not H12-treated mice laid significantly fewer eggs in vitro, and normal worm pairs incubated in vitro with the S13 mAb produced significantly fewer eggs than those incubated with H12 mAb. The impairment of egg hatching ability was also reproduced in vitro by the S13 mAb. These data suggest the existence of two different effector mechanisms induced by immunization with Sm 28 GST. The effect on the schistosome worm burden appears to be independent of GST activity whereas the effect on S. mansoni female fecundity and egg viability seems to be significantly linked to the inactivation of the enzymatic site.
研究了两种针对曼氏血吸虫28 kDa谷胱甘肽S-转移酶（Sm 28 GST）产生的不同单克隆抗体（mAb）的保护作用。根据对Sm 28 GST酶活性的阻断作用（S13）或缺乏阻断作用（H12），选择了两种相同亚型（IgM）的mAb。当被动转移到Fischer大鼠体内时，S13和H12均显著降低了虫负荷。在BALB/c小鼠中，转移S13 mAb时观察到对雌虫繁殖力和虫卵活力有明显影响；这些影响包括肠道虫卵负荷显著降低、虫卵孵化率降低以及死卵比例增加。在H12处理的小鼠中未观察到对产卵和虫卵孵化的影响。此外，从S13处理而非H12处理的小鼠中回收的虫对在体外产卵明显减少，并且在体外与S13 mAb孵育的正常虫对比与H12 mAb孵育的虫对产卵明显减少。S13 mAb在体外也重现了虫卵孵化能力的损害。这些数据表明，用Sm 28 GST免疫诱导存在两种不同的效应机制。对血吸虫虫负荷的影响似乎与GST活性无关，而对曼氏血吸虫雌虫繁殖力和虫卵活力的影响似乎与酶活性位点的失活显著相关。
