Percy A J, Harn D A
Department of Tropical Public Health, Harvard School of Public Health, Boston, MA 02115.
J Immunol. 1988 Apr 15;140(8):2760-2.
To study the role of idiotypic anti-idiotypic interactions in schistosomiasis, mice were immunized with a mAb, E.1, which bound to soluble egg and larval stage Ag and also passively transferred resistance to cercarial challenge in mice. Subsequently, hybridomas were produced from E.1 immunized mice and tested for the ability to inhibit E.1 binding to soluble egg Ag. The results showed that anti-idiotypic mAb (Ab2) were produced. The range of inhibitory activity was from 33 to 100%. By using a direct Ab2 binding assay, the Ab2 were shown to be idiotypic specific, not isotype specific. It was also found that six of the hybridomas bound to soluble egg Ag and were therefore anti-anti-idiotypic antibodies (Ab3). Ab3 were shown to be inhibited from binding to soluble egg Ag by Ab2. To the authors' knowledge, this is the first time that an in vivo network relevant to an infectious organism has been reproduced in vitro such that both Ab2 and Ab3 were produced from the same animals independent of exposure to parasite Ag.
为研究独特型抗独特型相互作用在血吸虫病中的作用，用一种单克隆抗体E.1免疫小鼠，该抗体可结合可溶性虫卵和幼虫期抗原，还能被动转移对小鼠尾蚴攻击的抵抗力。随后，从经E.1免疫的小鼠中制备杂交瘤，并检测其抑制E.1与可溶性虫卵抗原结合的能力。结果显示产生了抗独特型单克隆抗体（Ab2）。抑制活性范围为33%至100%。通过直接Ab2结合试验表明，Ab2具有独特型特异性，而非同种型特异性。还发现其中6个杂交瘤可结合可溶性虫卵抗原，因此是抗抗独特型抗体（Ab3）。Ab3被证明会被Ab2抑制与可溶性虫卵抗原的结合。据作者所知，这是首次在体外再现与感染性生物体相关的体内网络，使得Ab2和Ab3均由同一动物产生，且与暴露于寄生虫抗原无关。
