抗氧化剂对甲基胍产生羟自由基的抑制作用：一项电子自旋共振研究。

Inhibitory effect of antioxidants on hydroxyl radical generation from methylguanidine: an ESR study.

作者信息

Noda Yasuko, Mankura Mitsumasa

机构信息

Department of Anti-aging Food Sciences, Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama University, 2-5-1 Shikata-cho, Okayama, 700-8558, Japan.

出版信息

Neurochem Res. 2009 Apr;34(4):734-8. doi: 10.1007/s11064-008-9811-9. Epub 2008 Aug 8.

DOI:10.1007/s11064-008-9811-9

PMID:18688710

Abstract

Methylguanidine (MG) is known as not only a nephrotoxin but also as a neurotoxine. We have previously showed that MG itself generates hydroxyl radicals (*OH) in an in vitro study. In this study, we examined the inhibitory effects of ascorbate, EPC-K(1) (alpha-tocopheryl-L-ascorbate-2-O-phosphate diester), Trolox (water-soluble vitamin E analogue), and glutathione (GSH) on *OH generation from MG using an electron spin resonance (ESR) spectrometry with spin trap 5,5-dimethyl-1-pyrroline-N-oxide (DMPO). It was found that these compounds have potent inhibitory effect on *OH generation from MG in the order of ascorbate > GSH > EPC-K(1) > Trolox.

摘要

甲基胍（MG）不仅被认为是一种肾毒素，还被认为是一种神经毒素。我们之前在一项体外研究中表明，MG自身会产生羟基自由基（OH）。在本研究中，我们使用带有自旋捕捉剂5,5-二甲基-1-吡咯啉-N-氧化物（DMPO）的电子自旋共振（ESR）光谱法，研究了抗坏血酸、EPC-K(1)（α-生育酚-L-抗坏血酸-2-O-磷酸二酯）、Trolox（水溶性维生素E类似物）和谷胱甘肽（GSH）对MG产生OH的抑制作用。结果发现，这些化合物对MG产生*OH具有强大的抑制作用，其抑制作用顺序为抗坏血酸>GSH>EPC-K(1)>Trolox。