Cynshi O, Takashima Y, Katoh Y, Tamura K, Sato M, Fujita Y
Fujigotemba Research Laboratories Chugai Pharmaceutical Co., Ltd., Shizuoka, Japan.
J Biolumin Chemilumin. 1995 Sep-Oct;10(5):261-9. doi: 10.1002/bio.1170100502.
The action of phenolic antioxidants, such as probucol, on various active oxygen species was investigated using luminol chemiluminescence and spin trapping with 5,5-dimethyl-1-pyrroline-N-oxide (DMPO). The various active oxygen species, including hydroxyl radicals (Fenton reaction), superoxide anions, singlet oxygen and hypochlorite ions were examined with phenolic antioxidants under aqueous and nonaqueous conditions. Probucol showed a quenching effect on both superoxide anions and hypochlorite ions in nonaqueous solution. However, it had no effect on hydroxyl radicals. alpha-Tocopherol, a natural phenolic antioxidant, showed a stronger quenching effect on superoxide anions and hypochlorite ions than probucol, and quenched hydroxyl radicals in nonaqueous solution. Furthermore, Trolox showed a quenching effect on all active oxygen species in both aqueous and nonaqueous solution. The antioxidants were studied under comparable conditions in a series of test systems and the reactivity profiles depicted as 'radar charts' which are helpful for characterizing antioxidant action.
使用鲁米诺化学发光法以及5,5-二甲基-1-吡咯啉-N-氧化物（DMPO）自旋捕获技术，研究了酚类抗氧化剂如普罗布考对各种活性氧的作用。在水性和非水性条件下，用酚类抗氧化剂检测了包括羟基自由基（芬顿反应）、超氧阴离子、单线态氧和次氯酸根离子在内的各种活性氧。普罗布考在非水溶液中对超氧阴离子和次氯酸根离子均表现出猝灭作用。然而，它对羟基自由基没有影响。天然酚类抗氧化剂α-生育酚对超氧阴离子和次氯酸根离子的猝灭作用比普罗布考更强，并且在非水溶液中能猝灭羟基自由基。此外，Trolox在水性和非水性溶液中对所有活性氧均表现出猝灭作用。在一系列测试系统中，在可比条件下研究了这些抗氧化剂，并将反应活性谱描绘为“雷达图”，这有助于表征抗氧化作用。
