Jelski Wojciech, Kozlowski Miroslaw, Laudanski Jerzy, Niklinski Jacek, Szmitkowski Maciej
Department of Biochemical Diagnostics, Medical University, Waszyngtona 15 A, 15-269, Bialystok, Poland.
Dig Dis Sci. 2009 Apr;54(4):725-30. doi: 10.1007/s10620-008-0422-8. Epub 2008 Aug 9.
BACKGROUND/AIMS: Ethanol consumption is associated with an increased risk of esophageal cancer. The carcinogenic compound is acetaldehyde, the product of ethanol metabolism. Alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH) are the main enzymes involved in ethanol metabolism, which leads to generation of acetaldehyde. In this study the activity of ADH isoenzymes and ALDH in esophageal cancer were compared with the activity in normal tissue.
For measurement of the activity of class I and II ADH isoenzymes and ALDH activity fluorimetric methods were employed. Total ADH activity and activity of class III and IV isoenzymes was measured by the photometric method. Samples were taken from 59 esophageal cancer patients (27 adenocarcinoma, 32 squamous cell cancer).
The total activity of ADH and activity of class IV ADH were significantly higher in cancer cells than in healthy tissues. The other tested classes of ADH showed a tendency toward higher activity in cancer than in normal cells. Differences between the activity of enzymes of drinkers and non-drinkers in both cancer and healthy tissue were not significant.
Increased ADH IV activity may be a factor intensifying carcinogenesis, because of the increased ability to form acetaldehyde from ethanol.
背景/目的：饮酒与食管癌风险增加有关。致癌化合物是乙醛，它是乙醇代谢的产物。乙醇脱氢酶（ADH）和乙醛脱氢酶（ALDH）是参与乙醇代谢的主要酶，乙醇代谢会生成乙醛。在本研究中，对食管癌组织中ADH同工酶和ALDH的活性与正常组织中的活性进行了比较。
采用荧光法测定I类和II类ADH同工酶的活性以及ALDH的活性。采用光度法测定总ADH活性以及III类和IV类同工酶的活性。样本取自59例食管癌患者（27例腺癌，32例鳞状细胞癌）。
癌细胞中ADH的总活性和IV类ADH的活性显著高于健康组织。其他检测的ADH类在癌组织中的活性有高于正常细胞的趋势。饮酒者和不饮酒者在癌组织和健康组织中的酶活性差异不显著。
ADH IV活性增加可能是加剧致癌作用的一个因素，因为其将乙醇转化为乙醛的能力增强。
