Stahmer I, Zimmer J P, Ernst M, Fenner T, Finnern R, Schmitz H, Flad H D, Gerdes J
Forschungsinstitut Borstel, Department of Immunology and Cell Biology, FRG.
Eur J Immunol. 1991 Aug;21(8):1873-8. doi: 10.1002/eji.1830210814.
Immunohistological and electron microscopy studies of lymph nodes from patients infected with the human immunodeficiency virus 1 (HIV-1) demonstrated that follicular dendritic cells (FDC), the antigen-presenting cells of the B cell system, contain and may produce the virus. To elucidate the mode of infection of FDC with HIV-1 in vitro we developed an improved method for the preparation of single-cell suspensions of viable FDC with high purity (greater than 90% FDC). These isolated FDC were subjected to human T cell leukemia virus IIIB infection, which was monitored after 4 days in culture using the polymerase chain reaction. We were able to demonstrate that normal human FDC are highly susceptible to infection by HIV-1. Inhibition experiments with the monoclonal antibody OKT4a demonstrate that this infection is independent of the CD4 molecule.
对感染人类免疫缺陷病毒1（HIV-1）患者的淋巴结进行免疫组织学和电子显微镜研究表明，滤泡树突状细胞（FDC）作为B细胞系统的抗原呈递细胞，含有并可能产生该病毒。为了阐明体外FDC被HIV-1感染的模式，我们开发了一种改进方法，用于制备高纯度（FDC含量大于90%）的活性FDC单细胞悬液。将这些分离的FDC用人类T细胞白血病病毒IIIB感染，并在培养4天后使用聚合酶链反应进行监测。我们能够证明正常人类FDC对HIV-1感染高度敏感。用单克隆抗体OKT4a进行的抑制实验表明，这种感染不依赖于CD4分子。
