Smith O M, Gaffney D K, Anderson M S, McOlash L, Schober S L, Sieber F
Department of Pediatrics, Medical College of Wisconsin, Milwaukee 53226.
Exp Hematol. 1991 Sep;19(8):785-8.
Merocyanine 540 (MC 540) is a photosensitizing dye that has been used in a phase I clinical trial for the purging of leukemia and lymphoma cells from autologous bone marrow grafts. In this paper we examine the role of plasma membrane negative charge, plasma membrane fluidity, and plasma membrane hydrophobicity in the regulation of a cell's susceptibility to MC 540-sensitized photoirradiation. Among solid tumor cells, we found an inverse correlation between surface electronegativity, affinity for dye molecules, and susceptibility to MC 540-sensitized photoinactivation. That is, the least electronegative cells bound the highest amount of dye and were the most susceptible to dye-sensitized photoirradiation. By contrast, no such correlations were found among leukemia/lymphoma cells. This suggested that dye binding and susceptibility to MC 540-mediated photodynamic damages are regulated differently in hematopoietic/lymphopoietic and solid tumor cells.
部花青540（MC 540）是一种光敏染料，已用于一项一期临床试验，用于从自体骨髓移植物中清除白血病和淋巴瘤细胞。在本文中，我们研究了质膜负电荷、质膜流动性和质膜疏水性在调节细胞对MC 540致敏光照射敏感性中的作用。在实体瘤细胞中，我们发现表面电负性、对染料分子的亲和力与对MC 540致敏光灭活的敏感性之间呈负相关。也就是说，电负性最小的细胞结合的染料量最高，并且对染料致敏光照射最敏感。相比之下，在白血病/淋巴瘤细胞中未发现此类相关性。这表明造血/淋巴细胞和实体瘤细胞中染料结合和对MC 540介导的光动力损伤的敏感性受到不同的调节。
