Gaffney D K, Schober S L, Sieber F
Department of Biochemistry, Medical College of Wisconsin, Milwaukee.
Exp Hematol. 1990 Jan;18(1):23-6.
Merocyanine 540 (MC 540) is a photosensitizing dye that is used clinically for the purging of autologous bone marrow grafts and preclinically for the inactivation of enveloped viruses in blood products. In this paper we present evidence that the MC 540-sensitized photoinactivation of leukemia cells is an oxygen-dependent process and that unsaturated plasma membrane lipids are substrates for singlet oxygen and/or other activated oxygen species generated by photoirradiated MC 540. A comparison of the inhibition of clonal growth, the inhibition of mitochondrial respiration, and the exclusion of trypan blue by the plasma membrane after exposure to MC 540 and graded doses of light showed that mitochondrial respiration is compromised relatively early in the course of the dye-mediated photoinactivation of cells, well before the plasma membrane loses its capacity to exclude trypan blue. It also showed that trypan blue exclusion assays can greatly underestimate the cytotoxic effects of MC 540-sensitized photoirradiation.
部花青540（MC 540）是一种光敏染料，临床上用于净化自体骨髓移植，临床前用于灭活血液制品中的包膜病毒。在本文中，我们提供证据表明，MC 540致敏的白血病细胞光灭活是一个依赖氧气的过程，不饱和质膜脂质是单线态氧和/或光照射MC 540产生的其他活性氧物种的底物。比较暴露于MC 540和分级剂量光照后克隆生长的抑制、线粒体呼吸的抑制以及质膜对台盼蓝的排斥，结果表明，在染料介导的细胞光灭活过程中，线粒体呼吸相对较早受到损害，远在质膜失去排斥台盼蓝的能力之前。研究还表明，台盼蓝排斥试验可能会大大低估MC 540致敏光照射的细胞毒性作用。
