来自克里特岛和塞浦路斯的孕妇及患者中弓形虫的流行基因型。
Prevalent genotypes of Toxoplasma gondii in pregnant women and patients from Crete and Cyprus.
作者信息
Messaritakis Ippokratis, Detsika Maria, Koliou Maria, Sifakis Stavros, Antoniou Maria
机构信息
Laboratory of Clinical Bacteriology, Parasitology, Zoonoses and Geographical Medicine, Faculty of Medicine, University of Crete, Heraklion, Crete, Greece.
出版信息
Am J Trop Med Hyg. 2008 Aug;79(2):205-9.
Molecular genotyping has been used to characterize Toxoplasma gondii strains into the three clonal lineages known as types I, II, and III. To characterize T. gondii strains from Greece and Cyprus, polymerase chain reaction-restriction fragment length polymorphism analysis on the GRA6 gene was performed directly on 20 clinical samples from 18 humans (11 pregnant women, six patients with lymphadenopathy, and one patient positive for human immunodeficiency virus) and two rats. Characterization of T. gondii types was performed after digestion of amplified products with Mse I. The 20 strains were characterized as type II (20%) and type III (80%). Of these strains, 19 originated from the island of Crete (4 strains type II and 15 strains type III), and 1 from the island of Cyprus (type III). Although both type II and type III strains were found, type III was the most prevalent in Crete.
分子基因分型已被用于将弓形虫菌株分为三种克隆谱系，即I型、II型和III型。为了对来自希腊和塞浦路斯的弓形虫菌株进行特征分析，直接对来自18名人类（11名孕妇、6名淋巴结病患者和1名人类免疫缺陷病毒阳性患者）和2只大鼠的20份临床样本进行了GRA6基因的聚合酶链反应-限制性片段长度多态性分析。在用Mse I消化扩增产物后对弓形虫类型进行特征分析。这20株菌株被鉴定为II型（20%）和III型（80%）。在这些菌株中，19株来自克里特岛（4株II型和15株III型），1株来自塞浦路斯岛（III型）。虽然同时发现了II型和III型菌株，但III型在克里特岛最为普遍。
Zotero 插件