El-Sayad Mona Hassan, Salem Aziza Ibrahim, Fazary Hisham, Alzainny Haneen Nawaf, Abd El-Latif Naglaa Fathi
Department of Parasitology, Medical Research Institute, Alexandria University, 165, Horreya Avenue, Hadara, Alexandria, Egypt.
Department of Obstetrics and Gynaecology, Faculty of Medicine, Alexandria University, Alexandria, Egypt.
J Parasit Dis. 2021 Jun;45(2):539-545. doi: 10.1007/s12639-020-01327-0. Epub 2021 Jan 21.
Toxoplasmosis is a worldwide infection that can be acquired through the ingestion of tissue cysts in poorly cooked meat, and/or oblivious intake of sporulated oocysts in cat faeces, and transplacental. The infection in pregnant women is mainly asymptomatic. It produces abortion or congenital infection. The present study aimed to test the utility of polymerase chain reaction (PCR) on placental tissues in comparison to enzyme-linked immunosorbent assay (ELISA) to detect infections with in aborted women presented to Al-Shatby Maternity Hospital, Alexandria University, Egypt. Specific IgG and IgM were detected in serum by ELISA. Placental tissues from each participant were subjected to DNA extraction and PCR amplification. It was found that overall seroprevalence was 73%, DNA was detected in placenta tissues by using PCR analysis in 46% of cases. {× 2 () 18.124(< 0.001)}. Toxoplasma IgG/IgM by ELISA was positive in 23% of the cases, 20% showed amplified DNA by PCR. Positive IgG without IgM was seen in 27% cases, only 2% of them were positive by PCR. Moreover, positive PCR among positive ELISA IgM aborted women was 21 of the 23 cases. Positive PCR was obtained in three seronegative women. Our results showed that PCR sensitivity was 58.90 specificity 88.89, positive predictive value was 93.48%, and negative predictive value 44.44%. Although ELISA assay is still the gold standard of diagnosis of Toxoplasmosis, other diagnostic modalities are highly required particularly in those ELISA seronegative cases. PCR can be used as a sensitive and precise modality.
弓形虫病是一种全球性感染疾病，可通过摄入未煮熟肉类中的组织包囊和/或无意中摄入猫粪便中的孢子化卵囊以及经胎盘传播而获得。孕妇感染弓形虫病主要表现为无症状。它会导致流产或先天性感染。本研究旨在比较聚合酶链反应（PCR）与酶联免疫吸附测定（ELISA）检测埃及亚历山大大学阿尔-沙特比妇产医院流产女性弓形虫感染的效用。通过ELISA检测血清中的特异性IgG和IgM。对每位参与者的胎盘组织进行DNA提取和PCR扩增。结果发现总体血清阳性率为73%，通过PCR分析在46%的病例中检测到胎盘组织中有DNA。{× 2 () 18.124(< 0.001)}。ELISA检测弓形虫IgG/IgM阳性的病例占23%，PCR检测显示20%的病例DNA扩增。27%的病例IgG阳性而IgM阴性，其中只有2%通过PCR检测为阳性。此外ELISA IgM阳性的流产女性中PCR阳性的有23例中的21例。3例血清阴性女性PCR检测呈阳性。我们的结果显示PCR敏感性为58.90，特异性为88.89，阳性预测值为93.48%，阴性预测值为44.44%。虽然ELISA检测仍是弓形虫病诊断的金标准，但仍非常需要其他诊断方法，尤其是在那些ELISA血清阴性的病例中。PCR可作为一种灵敏且精确的检测方法。
