Lai Frances, Kazdhan Natasha, Lichty Brian D
Department of Medical Sciences, Michael G. DeGroote Institute for Infectious Disease Research, McMaster University, 1200 Main Street West, MDCL 5023, Hamilton, Ontario, Canada L8N 3Z5.
J Virol Methods. 2008 Nov;153(2):276-9. doi: 10.1016/j.jviromet.2008.07.001. Epub 2008 Aug 23.
A method is described for the use of a G-deleted, conditionally replicating version of vesicular stomatitis virus (VSV) to measure alterations to the innate anti-viral state of cells in vitro. By co-transfecting a gene of interest with an expression vector for VSV-G one can directly measure the replication of the virus in the transfected cells as non-transfected cells will fail to produce infectious progeny due to the lack of glycoprotein in these non-transfected cells. This allows the investigator to focus exclusively on the anti-viral state induced or inhibited in the transfected cells allowing for screening and quantitative analysis of viral or cellular genes that may modify the anti-viral state.
描述了一种使用G基因缺失的、条件性复制的水疱性口炎病毒（VSV）来体外测量细胞先天抗病毒状态改变的方法。通过将感兴趣的基因与VSV-G的表达载体共转染，可以直接测量病毒在转染细胞中的复制，因为未转染的细胞由于缺乏糖蛋白而无法产生感染性后代。这使得研究者能够专注于转染细胞中诱导或抑制的抗病毒状态，从而对可能改变抗病毒状态的病毒或细胞基因进行筛选和定量分析。
