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CD4与人类白细胞抗原II类抗原及HIV gp120之间的相互作用。

Interaction of CD4 with HLA class II antigens and HIV gp120.

作者信息

Piatier-Tonneau D, Gastinel L N, Amblard F, Wojcik M, Vaigot P, Auffray C

机构信息

Institut d'Embryologie Cellulaire et Moléculaire, CNRS, Collège de France, Nogent-sur-Marne.

出版信息

Immunogenetics. 1991;34(2):121-8. doi: 10.1007/BF00211424.

DOI:10.1007/BF00211424
PMID:1869305
Abstract

We have developed a cellular adhesion assay in which B lymphocytes expressing HLA class II antigens form rosettes with COS cells expressing high levels of cell surface CD4 upon transient transfection with a CDM8-CD4 plasmid construct. The assay is specific, quantitative, and overcomes the difficulties encountered with a previously described system using an SV40 viral vector. Rosette formation was inhibited by a series of CD4- and HLA-DR-specific antibodies, as well as by human immunodeficiency virus (HIV) gp 120, and a synthetic peptide derived from part of its binding site for CD4 (amino acid residues 414-434), but not by a variety of other effectors, including several soluble CD4 derivatives. The comparison of this pattern of inhibition with those observed in other systems further emphasizes the great similarity, but incomplete identity, in the CD4 binding sites for HLA class II antigens and HIV gp120, and supports a model in which CD4 is considered as an allosteric servomodulator of T-cell adhesion and function which probably is induced to interact with HLA class II antigens when associated with the Tcr/CD3 complex.

摘要

我们开发了一种细胞黏附试验，其中表达HLA II类抗原的B淋巴细胞与通过用CDM8-CD4质粒构建体瞬时转染而表达高水平细胞表面CD4的COS细胞形成玫瑰花结。该试验具有特异性、定量性，并且克服了使用SV40病毒载体的先前描述系统所遇到的困难。玫瑰花结的形成受到一系列CD4和HLA-DR特异性抗体的抑制，也受到人类免疫缺陷病毒（HIV）gp120以及源自其CD4结合位点一部分（氨基酸残基414-434）的合成肽的抑制，但不受包括几种可溶性CD4衍生物在内的多种其他效应物的抑制。将这种抑制模式与在其他系统中观察到的模式进行比较，进一步强调了HLA II类抗原和HIV gp120的CD4结合位点具有很大的相似性，但并非完全相同，并支持一种模型，其中CD4被认为是T细胞黏附和功能的变构伺服调节因子，当与Tcr/CD3复合物相关联时，可能被诱导与HLA II类抗原相互作用。

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本文引用的文献

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Studies on cell adhesion and recognition. II. The kinetics of cell adhesion and cell spreading on surfaces coated with carbohydrate-reactive proteins (glycosidases and lectins) and fibronectin.细胞黏附与识别研究。II. 细胞在涂有碳水化合物反应蛋白（糖苷酶和凝集素）及纤连蛋白的表面上的黏附动力学和细胞铺展。
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Human B cell variants immunoselected against a single Ia antigen subset have lost expression of several Ia antigen subsets.
针对单个Ia抗原亚群进行免疫选择的人B细胞变体已失去了几个Ia抗原亚群的表达。
J Exp Med. 1983 Mar 1;157(3):1053-8. doi: 10.1084/jem.157.3.1053.
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Cell-cell adhesion mediated by CD8 and MHC class I molecules.由CD8和MHC I类分子介导的细胞间粘附。
Nature. 1988 Nov 3;336(6194):79-81. doi: 10.1038/336079a0.
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Immunosuppressive properties of synthetic peptides derived from CD4 and HLA-DR antigens.
Cell. 1988 Nov 4;55(3):497-504. doi: 10.1016/0092-8674(88)90036-0.
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Stimulation and proliferation of CD4+ peripheral blood T lymphocytes induced by an anti-CD4 monoclonal antibody.
Eur J Immunol. 1988 Mar;18(3):333-9. doi: 10.1002/eji.1830180303.
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A soluble form of CD4 (T4) protein inhibits AIDS virus infection.可溶性形式的CD4（T4）蛋白可抑制艾滋病病毒感染。
Nature. 1988 Jan 7;331(6151):82-4. doi: 10.1038/331082a0.
8
Identification of homologous regions in human immunodeficiency virus I gp41 and human MHC class II beta 1 domain. I. Monoclonal antibodies against the gp41-derived peptide and patients' sera react with native HLA class II antigens, suggesting a role for autoimmunity in the pathogenesis of acquired immune deficiency syndrome.人类免疫缺陷病毒I型gp41与人MHC II类β1结构域同源区域的鉴定。I. 针对gp41衍生肽的单克隆抗体和患者血清与天然HLA II类抗原发生反应，提示自身免疫在获得性免疫缺陷综合征发病机制中起作用。
J Exp Med. 1988 Mar 1;167(3):914-23. doi: 10.1084/jem.167.3.914.
9
Functional interaction between human T-cell protein CD4 and the major histocompatibility complex HLA-DR antigen.人类T细胞蛋白CD4与主要组织相容性复合体HLA-DR抗原之间的功能相互作用。
Nature. 1987;328(6131):626-9. doi: 10.1038/328626a0.
10
The role of the T cell receptor, CD8, and LFA-1 in different stages of the cytolytic reaction mediated by alloreactive T lymphocyte clones.T细胞受体、CD8和淋巴细胞功能相关抗原-1在同种异体反应性T淋巴细胞克隆介导的细胞溶解反应不同阶段中的作用。
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