Alayli Gamze, Kara Nurten, Tander Berna, Canturk Ferhan, Gunes Sezgin, Bagci Hasan
Department of Physical Medicine and Rehabilitation, Ondokuz Mayis University, 55139 Kurupelit, Samsun, Turkey.
Joint Bone Spine. 2009 Jan;76(1):20-3. doi: 10.1016/j.jbspin.2008.02.012. Epub 2008 Aug 6.
Cytokine genes play important roles in the pathogenesis of rheumatoid arthritis (RA). In RA, the plasma and synovial fluid levels of transforming growth factor beta1 (TGFbeta1) have been shown to be raised. The aim of this study was to investigate the relationship between the TGFbeta1 T869C polymorphism and RA in a Turkish population.
One hundred and thirty-one patients with a clinical diagnosis of RA and 133 healthy controls were enrolled in this study. Analyses of TGFbeta1 T869C gene were made by the polymerase chain reaction-restriction fragment length polymorphism technique.
There was no significant difference in genotypic frequency of TGFbeta1 T869C polymorphism between the patients with RA (TT:TC:CC=42.7%:41.2%:16%) and controls (TT:TC:CC=36.1%:48.1%:15.8%) (p=0.48). The age at first occurrence of clinical symptoms of RA did not differ significantly in relation to TGFbeta1 T869C genotypes (p=0.07). Furthermore, there was no significant association between TGFbeta1 T869C genotypes and the presence or absence of radiographic erosions in the patient group (p=0.67). But presence of T allele was associated with 1.92-fold increased risk for RF positivity (p=0.02, OR=1.92, 95% CI=1.08-3.40).
The allele frequencies for TGFbeta1 T869C polymorphism in RA patients were similar to those in the control group. However, the T allele carriers had 1.92-fold increased risk for RF positivity. Further studies on larger numbers of cases and on the other polymorphic regions of this gene are needed before definite conclusions can be drawn about the role of TGFbeta1 in the etiology of RA.
细胞因子基因在类风湿关节炎（RA）的发病机制中起重要作用。在RA中，已表明转化生长因子β1（TGFβ1）的血浆和滑液水平升高。本研究的目的是调查土耳其人群中TGFβ1 T869C多态性与RA之间的关系。
本研究纳入了131例临床诊断为RA的患者和133例健康对照。采用聚合酶链反应-限制性片段长度多态性技术对TGFβ1 T869C基因进行分析。
RA患者（TT:TC:CC = 42.7%:41.2%:16%）和对照组（TT:TC:CC = 36.1%:48.1%:15.8%）之间TGFβ1 T869C多态性的基因型频率无显著差异（p = 0.48）。RA临床症状首次出现的年龄与TGFβ1 T869C基因型无显著差异（p = 0.07）。此外，患者组中TGFβ1 T869C基因型与影像学侵蚀的有无之间无显著关联（p = 0.67）。但T等位基因的存在与RF阳性风险增加1.92倍相关（p = 0.02，OR = 1.92，95% CI = 1.08 - 3.40）。
RA患者中TGFβ1 T869C多态性的等位基因频率与对照组相似。然而，T等位基因携带者RF阳性风险增加1.92倍。在就TGFβ1在RA病因学中的作用得出明确结论之前，需要对更多病例以及该基因的其他多态性区域进行进一步研究。
