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2
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本文引用的文献

1
Survival of mycobacteria in macrophages is mediated by coronin 1-dependent activation of calcineurin.巨噬细胞中分枝杆菌的存活是由钙调神经磷酸酶的冠蛋白1依赖性激活介导的。
Cell. 2007 Jul 13;130(1):37-50. doi: 10.1016/j.cell.2007.04.043.
2
Coronin function is required for chemotaxis and phagocytosis in human neutrophils.冠蛋白功能是人类中性粒细胞趋化性和吞噬作用所必需的。
J Immunol. 2007 May 1;178(9):5769-78. doi: 10.4049/jimmunol.178.9.5769.
3
Requirement for coronin 1 in T lymphocyte trafficking and cellular homeostasis.T淋巴细胞迁移和细胞稳态中冠蛋白1的需求。
Science. 2006 Aug 11;313(5788):839-42. doi: 10.1126/science.1130563.
4
Coronins: the return of the crown.冠蛋白：皇冠的回归。
Trends Cell Biol. 2006 Aug;16(8):421-6. doi: 10.1016/j.tcb.2006.06.002. Epub 2006 Jun 27.
5
Phosphorylation of coronin 1B by protein kinase C regulates interaction with Arp2/3 and cell motility.蛋白激酶C对冠蛋白1B的磷酸化作用调节其与肌动蛋白相关蛋白2/3复合物的相互作用及细胞运动。
J Biol Chem. 2005 Sep 9;280(36):31913-23. doi: 10.1074/jbc.M504146200. Epub 2005 Jul 18.
6
Coronin-1 function is required for phagosome formation.吞噬体形成需要冠蛋白-1发挥功能。
Mol Biol Cell. 2005 Jul;16(7):3077-87. doi: 10.1091/mbc.e04-11-0989. Epub 2005 Apr 13.
7
Association of the leukocyte plasma membrane with the actin cytoskeleton through coiled coil-mediated trimeric coronin 1 molecules.通过卷曲螺旋介导的三聚体冠蛋白1分子使白细胞质膜与肌动蛋白细胞骨架发生关联。
Mol Biol Cell. 2005 Jun;16(6):2786-98. doi: 10.1091/mbc.e05-01-0042. Epub 2005 Mar 30.
8
Homotypic dimerization of the actin-binding protein p57/coronin-1 mediated by a leucine zipper motif in the C-terminal region.由C端区域的亮氨酸拉链基序介导的肌动蛋白结合蛋白p57/冠蛋白-1的同型二聚化。
Biochem J. 2005 Apr 15;387(Pt 2):325-31. doi: 10.1042/BJ20041020.
9
Disruption of mptpB impairs the ability of Mycobacterium tuberculosis to survive in guinea pigs.mptpB基因的破坏会损害结核分枝杆菌在豚鼠体内的生存能力。
Mol Microbiol. 2003 Nov;50(3):751-62. doi: 10.1046/j.1365-2958.2003.03712.x.
10
Two regions responsible for the actin binding of p57, a mammalian coronin family actin-binding protein.负责p57（一种哺乳动物冠蛋白家族肌动蛋白结合蛋白）肌动蛋白结合的两个区域。
Biol Pharm Bull. 2003 Apr;26(4):409-16. doi: 10.1248/bpb.26.409.

佛波酯依赖性磷酸化调节p57/冠蛋白-1与肌动蛋白细胞骨架的结合。

Phorbol ester-dependent phosphorylation regulates the association of p57/coronin-1 with the actin cytoskeleton.

作者信息

Oku Teruaki, Kaneko Yutaka, Murofushi Koki, Seyama Yoshiyuki, Toyoshima Satoshi, Tsuji Tsutomu

机构信息

Department of Microbiology, Hoshi University School of Pharmacy and Pharmaceutical Sciences, Tokyo 142-8501, Japan.

出版信息

J Biol Chem. 2008 Oct 24;283(43):28918-25. doi: 10.1074/jbc.M709990200. Epub 2008 Aug 7.

DOI:10.1074/jbc.M709990200
PMID:18693254
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2662003/
Abstract

The p57/coronin-1 protein is a member of the coronin family of actin-binding proteins, which are characterized by the presence of WD (tryptophan/aspartic acid) repeats and a coiled-coil motif in the molecule. It is selectively expressed in immune cells and has been suggested to play crucial roles in leukocyte functions, including cell migration and phagocytosis. In this study we examined the effects of p57/coronin-1 phosphorylation on the association of the protein with actin. Treatment of HL60 human leukemic cells or p57/coronin-1-transfected HEK293 cells with phorbol 12-myristate 13-acetate (PMA) reduced the association of p57/coronin-1 with the actin cytoskeleton, as indicated by cell fractionation experiments and by fluorescence microscopic observation. Two-dimensional gel electrophoresis of HL60 cell lysate revealed that p57/coronin-1 was phosphorylated upon PMA stimulation of the cells, giving two major and two minor spots of phosphorylated forms, each with distinct isoelectric points. The p57/coronin-1 molecules associated with the cytoskeleton in PMA-treated HL60 cells were phosphorylated at lower levels than those recovered in the cytosolic fraction. In addition, p57/coronin-1 co-sedimented with F-actin polymerized in vitro had lower phosphorylation levels than the molecules remaining in the supernatant. By affinity chromatographic analysis using anti-p57/coronin-1 antibody-conjugated Sepharose, p57/coronin-1 derived from PMA-treated HL60 cells showed lower affinity for actin than that from untreated cells. Finally, recovery of p57/coronin-1 in the actin cytoskeleton-rich fraction from neutrophil-like differentiated HL60 cells decreased during phagocytosis, concomitant with enhanced phosphorylation of p57/coronin-1. These results strongly suggest that the phosphorylation of p57/coronin-1 down-regulates its association with actin and modulates the reorganization of actin-containing cytoskeleton.

摘要

p57/冠蛋白-1蛋白是肌动蛋白结合蛋白冠蛋白家族的成员，其分子特征是存在WD（色氨酸/天冬氨酸）重复序列和卷曲螺旋基序。它在免疫细胞中选择性表达，并被认为在白细胞功能中起关键作用，包括细胞迁移和吞噬作用。在本研究中，我们检测了p57/冠蛋白-1磷酸化对该蛋白与肌动蛋白结合的影响。用佛波酯12-肉豆蔻酸酯13-乙酸酯（PMA）处理HL60人白血病细胞或p57/冠蛋白-1转染的HEK293细胞，细胞分级分离实验和荧光显微镜观察表明，这降低了p57/冠蛋白-1与肌动蛋白细胞骨架的结合。HL60细胞裂解物的二维凝胶电泳显示，PMA刺激细胞后p57/冠蛋白-1发生磷酸化，产生两个主要和两个次要的磷酸化形式斑点，每个斑点具有不同的等电点。与PMA处理的HL60细胞中细胞骨架相关的p57/冠蛋白-1分子的磷酸化水平低于在细胞质部分中回收的分子。此外，与体外聚合的F-肌动蛋白共沉降的p57/冠蛋白-1的磷酸化水平低于上清液中剩余的分子。通过使用抗p57/冠蛋白-1抗体偶联的琼脂糖进行亲和色谱分析，来自PMA处理的HL60细胞的p57/冠蛋白-1对肌动蛋白的亲和力低于未处理细胞的p57/冠蛋白-1。最后，在吞噬作用期间，来自嗜中性粒细胞样分化的HL60细胞的富含肌动蛋白细胞骨架部分中p57/冠蛋白-1的回收率降低，同时p57/冠蛋白-1的磷酸化增强。这些结果强烈表明，p57/冠蛋白-1的磷酸化下调其与肌动蛋白的结合并调节含肌动蛋白细胞骨架的重组。