Sharoian S G, Shaldzhian A A, Nalbandian R M, Buniatian G Kh
Vopr Biokhim Mozga. 1975;10:66-74.
Cytochrome c from grey matter of brain has been obtained as a homogeneous preparation by electrophoresis on polyacrylamide gel and following electrofocusing in ampholine solutions. Its molecular weight, content of iron, redox potential and isoelectric point have been established. The absolute spectra of its oxidized and reduced forms are presented. Cytochrome c of brain cortex is similar in its properties to that obtained from other animal tissues as the heart and adrenal cortex.
通过在聚丙烯酰胺凝胶上电泳以及随后在两性电解质溶液中进行电聚焦，已获得来自脑灰质的细胞色素c的纯品。已确定其分子量、铁含量、氧化还原电位和等电点。给出了其氧化态和还原态的绝对光谱。大脑皮质的细胞色素c在性质上与从心脏和肾上腺皮质等其他动物组织中获得的细胞色素c相似。
