Takakuwa S
J Biochem. 1975 Jul;78(1):181-5.
A soluble cytochrome c-552 from Thiobacillus thiooxidans was highly purified and its physico-chemical properteis were studied. The absorption maxima were at 552,523,418 nm in the reduced from and at 412 nm in the oxidized form. The pyridine hemochrome spectrum was the same as that of other cytochromes c. The molecular weight, estimated by the gel filtration method, was found to be 12,600. The isoelectric point was determined to be 9.2-9.3 by the electrofocusing technique. The standard oxidation-reduction potential of this cytochrome was +0.247 V.
对氧化硫硫杆菌中的一种可溶性细胞色素c-552进行了高度纯化，并对其理化性质进行了研究。还原态时的吸收峰最大值分别在552、523、418nm处，氧化态时在412nm处。吡啶血色原光谱与其他细胞色素c相同。通过凝胶过滤法估算的分子量为12,600。采用电聚焦技术测定其等电点为9.2 - 9.3。该细胞色素的标准氧化还原电位为+0.247V。
