Anastassopoulou C G, Kafatos G, Nardone A, Andrews N, Pebody R G, Mossong J, Davidkin I, Gelb D, DE Ory F, Thierfelder W, Nemecek V, Bruzzone B, Butur D, Barbara C, Sobotová Z, Jones L, Griskevicius A, Hesketh L M, Cohen D, Vranckx R, Tsakris A, Miller E, Hatzakis A
Department of Hygiene and Epidemiology, University of Athens Medical School, Athens, Greece.
Epidemiol Infect. 2009 Apr;137(4):485-94. doi: 10.1017/S0950268808001155. Epub 2008 Aug 11.
The European Sero-Epidemiology Network 2 (ESEN2) aimed to compare serological results of vaccine-preventable diseases across Europe. To ensure direct inter-country comparability of hepatitis A virus antibody (anti-HAV) measurements, a standardization panel of 150 sera was developed by a designated reference laboratory and tested by participating national laboratories using assays of choice; each country's results were subsequently regressed against those of the reference laboratory. Quantitatively, the assays were generally highly correlated (R2>0.90). Nevertheless, qualitative comparisons indicated that results obtained with different assays may differ despite the usage of well-established international and local standards. To a great extent standardization successfully alleviated such differences. The generated standardization equations will be used to convert national serological results into common units to enable direct international comparisons of HAV seroprevalence data. The results of this study are expected to contribute to the evaluation and potential improvement of the currently employed immunization strategies for hepatitis in Europe.
欧洲血清流行病学网络2（ESEN2）旨在比较欧洲各国疫苗可预防疾病的血清学结果。为确保甲型肝炎病毒抗体（抗-HAV）检测结果在不同国家之间具有直接可比性，指定的参考实验室开发了一个由150份血清组成的标准化样本，并由参与的国家实验室使用各自选择的检测方法进行检测；随后将每个国家的检测结果与参考实验室的结果进行回归分析。在定量方面，这些检测方法通常具有高度相关性（R2>0.90）。然而，定性比较表明，尽管使用了成熟的国际和当地标准，但不同检测方法获得的结果仍可能存在差异。在很大程度上，标准化成功地缓解了这些差异。生成的标准化方程将用于将各国的血清学结果转换为通用单位，以便直接进行甲型肝炎血清流行率数据的国际比较。预计本研究结果将有助于评估和潜在改进欧洲目前采用的肝炎免疫策略。
