Casal G, Matos E, Teles-Grilo M L, Azevedo C
Department of Cell Biology, Institute of Biomedical Sciences, University of Porto (ICBAS/UP), Lg. A. Salazar no. 2, P-4099-003 Porto, Portugal.
Parasitology. 2008 Aug;135(9):1053-64. doi: 10.1017/S0031182008004654.
A fish-infecting Microsporidia Potaspora morhaphis n. gen., n. sp. found adherent to the wall of the coelomic cavity of the freshwater fish, Potamorhaphis guianensis, from lower Amazon River is described, based on light microscope and ultrastructural characteristics. This microsporidian forms whitish xenomas distinguished by the numerous filiform and anastomosed microvilli. The xenoma was completely filled by several developmental stages. In all of these stages, the nuclei are monokaryotic and develop in direct contact with host cell cytoplasm. The merogonial plasmodium divides by binary fission and the disporoblastic pyriform spores of sporont origin measure 2.8+/-0.3 x 1.5+/-0.2 microm. In mature spores the polar filament was arranged into 9-10 coils in 2 layers. The polaroplast had 2 distinct regions around the manubrium and an electron-dense globule was observed. The small subunit, intergenic space and partial large subunit rRNA gene were sequenced and maximum parsimony analysis placed the microsporidian described here in the clade that includes the genera Kabatana, Microgemma, Spraguea and Tetramicra. The ultrastructural morphology of the xenoma, and the developmental stages including the spores of this microsporidian parasite, as well as the phylogenetic analysis, suggest the erection of a new genus and species.
基于光学显微镜和超微结构特征，描述了一种寄生于淡水鱼圭亚那河波塔莫拉菲斯（Potamorhaphis guianensis）体腔壁上的感染鱼类的微孢子虫——波塔斯孢虫属（Potaspora）新属新种。这种微孢子虫形成白色的孢囊瘤，其特征是有许多丝状且相互吻合的微绒毛。孢囊瘤完全被几个发育阶段所充满。在所有这些阶段中，细胞核均为单核，并与宿主细胞质直接接触发育。裂殖体通过二分裂进行分裂，孢子母细胞起源的双孢子型梨形孢子大小为2.8±0.3×1.5±0.2微米。在成熟孢子中，极丝呈两层排列，共9 - 10圈。极质体在柄周围有两个不同区域，并观察到一个电子致密小球。对小亚基、基因间隔区和部分大亚基rRNA基因进行了测序，最大简约分析将此处描述的微孢子虫置于包含卡巴塔纳属（Kabatana）、微宝石属（Microgemma）、斯普拉格属（Spraguea）和四微孢属（Tetramicra）的进化枝中。孢囊瘤的超微结构形态、该微孢子虫寄生虫的发育阶段（包括孢子）以及系统发育分析，都表明应建立一个新属和新种。
