Pearl River Fishery Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, Guangdong 510380, China.
Parasitol Res. 2012 Oct;111(4):1715-24. doi: 10.1007/s00436-012-3013-8. Epub 2012 Jul 8.
A potentially fatal microsporidial infection targeting the skeletal muscles of the tiger barb Puntius tetrazona was described. Ultrastructural and molecular analyses of infected tissues confirmed that the causative parasite was Pleistophora hyphessobryconis. Compared to P. hyphessobryconis observed in other hosts, those infecting tiger barb demonstrated differences in ultrastructure that may be related to host adaptation. Phylogenetic analysis revealed that classifications based on different methods of analysis (molecular, morphologic, or developmental) do not always coincide, and suggesting that the genetic relationships between Pleistophora and Ovipleistophora may need to be redefined. Transparent mutants of tiger barb can be artificially infected by P. hyphessobryconis, and the dynamic process and spatial distribution of P. hyphessobryconis infection can be observed in real time. These transparent fish mutants are a valuable model to study microsporidial infection in vivo.
描述了一种针对虎皮鱼（Puntius tetrazona）骨骼肌的潜在致命微孢子虫感染。对感染组织的超微结构和分子分析证实，致病寄生虫是 Hyphessobrycon 微孢子虫。与在其他宿主中观察到的 Hyphessobrycon 微孢子虫相比，感染虎皮鱼的寄生虫在超微结构上存在差异，这可能与宿主适应有关。系统发育分析表明，基于不同分析方法（分子、形态或发育）的分类并不总是一致的，并表明 Pleistophora 和 Ovipleistophora 之间的遗传关系可能需要重新定义。虎皮鱼的透明突变体可以被 Hyphessobrycon 微孢子虫人工感染，并且可以实时观察 Hyphessobrycon 微孢子虫感染的动态过程和空间分布。这些透明鱼突变体是研究体内微孢子虫感染的有价值的模型。
