Wozniak A C, Anderson J E
Department of Human Anatomy and Cell Science, Faculty of Medicine, The University of Manitoba, Canada.
Int J Biochem Cell Biol. 2009 Mar;41(3):625-31. doi: 10.1016/j.biocel.2008.07.005. Epub 2008 Jul 25.
Potent nitric oxide (NO) signals are described for many forms of cell-cell communication. Although NO plays a significant role in skeletal muscle metabolism and contractility and in precursor activation during muscle formation and stretching, there is no direct evidence of stretch-induced NO release from muscle. Differentiated muscle cell cultures from normal and dystrophic mdx mice were preloaded with the NO-specific dye DAF-2 (diaminofluorescein-2) before stretching. NO release was detected by video-microscopy. NO was released rapidly from wild-type (WT) cells after stretch and intensity declined rapidly to a plateau. Mdx cells showed much less NO release. Direct observations of the time-course of stretch-induced NO release in WT cells is congruent with the hypothesis of NO-mediated stretch activation of satellite cells in normal skeletal muscle. Distinct differences in the time-course between normal and dystrophic cells indicate visualization methods for NO release will be a sensitive measure of NOS-1 restoration following diverse treatment approaches to muscular dystrophy.
强效一氧化氮（NO）信号在多种细胞间通讯形式中均有描述。尽管NO在骨骼肌代谢与收缩以及肌肉形成和拉伸过程中的前体细胞激活中发挥着重要作用，但尚无直接证据表明拉伸会诱导肌肉释放NO。在拉伸前，将来自正常和营养不良的mdx小鼠的分化肌肉细胞培养物预先加载NO特异性染料DAF-2（二氨基荧光素-2）。通过视频显微镜检测NO释放。拉伸后，野生型（WT）细胞迅速释放NO，其强度迅速下降至平稳状态。mdx细胞释放的NO则少得多。对WT细胞中拉伸诱导的NO释放时间进程的直接观察结果与正常骨骼肌中卫星细胞的NO介导的拉伸激活假说一致。正常细胞和营养不良细胞在时间进程上的明显差异表明，NO释放的可视化方法将是对多种肌肉营养不良治疗方法后NOS-1恢复情况的敏感指标。
