Iakovleva L S, Zueva Iu N, Pavlovskaia A I, Mazurenko N P, Tsvetkov V S
Vopr Onkol. 1976;22(7):76-81.
While infecting VMD-Kekava chick fibroblasts cultures two plaque-forming variants of the virus were isolated. Both variants are cell-associated viruses; during a prolonged period these are circulating in the blood of infected chicks, including also healthy ones. The cultures infected contain a common precipitating antigen; sera from the infected chicks have antibodies to VMD feather antigen, both viruses produced, although in a different degree, in the infected chicks lymphoid infiltration of nerve trunks typical for Marek's disease. These variants differ one from the other in the type of plaques formed by them in the tissue culture, and in their pathogenicity for chicks. Variant 83 is likely to be naturally attenuated virus, and further on it may be investigated as a vaccine strain.
在感染VMD - 凯卡瓦鸡胚成纤维细胞培养物时，分离出了该病毒的两种形成蚀斑的变体。这两种变体都是与细胞相关的病毒；在较长一段时间内，它们在受感染雏鸡的血液中循环，包括健康雏鸡的血液。受感染的培养物含有一种共同的沉淀抗原；受感染雏鸡的血清对VMD羽毛抗原有抗体，两种病毒在受感染雏鸡中均产生了马立克氏病典型的神经干淋巴浸润，不过程度不同。这些变体在组织培养中形成的蚀斑类型以及对雏鸡的致病性方面彼此不同。变体83可能是自然减毒病毒，进一步可将其作为疫苗株进行研究。
