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豆科植物凝集素FRIL在体外悬浮培养中可保存神经祖细胞。

Legume lectin FRIL preserves neural progenitor cells in suspension culture in vitro.

作者信息

Yao Hailei, Xie Xiaoyan, Li Yanhua, Wang Dongmei, Han Shu, Shi Shuangshuang, Nan Xue, Bai Cixian, Wang Yunfang, Pei Xuetao

机构信息

Stem Cells and Regenerative Medicine Lab, Beijing Institute of Transfusion Medicine, Beijing 100850, China.

出版信息

Clin Dev Immunol. 2008;2008:531317. doi: 10.1155/2008/531317.

DOI:10.1155/2008/531317
PMID:18695740
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2496955/
Abstract

In vitro maintenance of stem cells is crucial for many clinical applications. Stem cell preservation factor FRIL (Flt3 receptor-interacting lectin) is a plant lectin extracted from Dolichos Lablab and has been found preserve hematopoietic stem cells in vitro for a month in our previous studies. To investigate whether FRIL can preserve neural progenitor cells (NPCs), it was supplemented into serum-free suspension culture media. FRIL made NPC grow slowly, induced cell adhesion, and delayed neurospheres formation. However, FRIL did not initiate NPC differentiation according to immunofluorescence and semiquantitive RT-PCR results. In conclusion, FRIL could also preserve neural progenitor cells in vitro by inhibiting both cell proliferation and differentiation.

摘要

干细胞的体外维持对于许多临床应用至关重要。干细胞保护因子FRIL（Flt3受体相互作用凝集素）是一种从白扁豆中提取的植物凝集素，在我们之前的研究中已发现它能在体外保存造血干细胞长达一个月。为了研究FRIL是否能保存神经祖细胞（NPCs），将其添加到无血清悬浮培养基中。FRIL使NPCs生长缓慢，诱导细胞黏附，并延迟神经球形成。然而，根据免疫荧光和半定量RT-PCR结果，FRIL并未引发NPCs分化。总之，FRIL还可通过抑制细胞增殖和分化在体外保存神经祖细胞。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9922/2496955/70adeda31556/CDI2008-531317.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9922/2496955/874e51f65389/CDI2008-531317.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9922/2496955/b170af994587/CDI2008-531317.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9922/2496955/2a579ab3dd02/CDI2008-531317.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9922/2496955/9dfb63b53025/CDI2008-531317.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9922/2496955/70adeda31556/CDI2008-531317.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9922/2496955/874e51f65389/CDI2008-531317.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9922/2496955/b170af994587/CDI2008-531317.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9922/2496955/2a579ab3dd02/CDI2008-531317.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9922/2496955/9dfb63b53025/CDI2008-531317.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9922/2496955/70adeda31556/CDI2008-531317.005.jpg

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[The mechanisms of a new plant lectin FRIL from dolichos lablab that preserves quiescent hematopoietic stem cells in suspension culture].[一种来自白扁豆的新型植物凝集素FRIL在悬浮培养中保存静止造血干细胞的机制]
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