YDJ1的特性：细菌dnaJ蛋白的酵母同源物。
Characterization of YDJ1: a yeast homologue of the bacterial dnaJ protein.
作者信息
Caplan A J, Douglas M G
机构信息
Department of Biochemistry and Biophysics, University of North Carolina, Chapel Hill 27599-7260.
出版信息
J Cell Biol. 1991 Aug;114(4):609-21. doi: 10.1083/jcb.114.4.609.
Abstract
The YDJ1 (yeast dnaJ) gene was isolated from a yeast expression library using antisera made against a yeast nuclear sub-fraction termed the matrix lamina pore complex. The predicted open reading frame displays a 32% identity with the sequence of the Escherichia coli heat shock protein dnaJ. Localization of YDJ1 protein (YDJ1p) by indirect immunofluorescence reveals it to be concentrated in a perinuclear ring as well as in the cytoplasm. YDJ1p cofractionates with nuclei and also microsomes, suggesting that its perinuclear localization reflects association with the ER. YDJ1p is required for normal growth and disruption of its gene results in very slow growing cells that have pleiotropic morphological defects. Haploid cells carrying the disrupted YDJ1 gene are inviable for growth in liquid media. We further show that a related yeast protein, SIS1, is a multicopy suppressor of YDJ1.
摘要
YDJ1（酵母dnaJ）基因是从酵母表达文库中分离出来的，所用抗血清是针对一种称为核基质层孔复合体的酵母核亚组分制备的。预测的开放阅读框与大肠杆菌热休克蛋白dnaJ的序列有32%的同一性。通过间接免疫荧光对YDJ1蛋白（YDJ1p）进行定位，发现它集中在核周环以及细胞质中。YDJ1p与细胞核以及微粒体共分级，这表明其核周定位反映了与内质网的关联。YDJ1p是正常生长所必需的，其基因的破坏会导致细胞生长非常缓慢，并具有多效性形态缺陷。携带被破坏的YDJ1基因的单倍体细胞在液体培养基中无法生长。我们进一步表明，一种相关的酵母蛋白SIS1是YDJ1的多拷贝抑制因子。
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