大肠杆菌DNAJ热休克蛋白独特人类同源物的克隆

Cloning of a unique human homologue of the Escherichia coli DNAJ heat shock protein.

作者信息

Chellaiah A, Davis A, Mohanakumar T

机构信息

Department of Surgery and Pathology, Washington University School of Medicine, St. Louis, MO 63110.

出版信息

Biochim Biophys Acta. 1993 Jul 18;1174(1):111-3. doi: 10.1016/0167-4781(93)90103-k.

DOI:10.1016/0167-4781(93)90103-k

PMID:8334160

Abstract

A human homologue of the bacterial DNAJ heatshock protein, HDJ-2, was isolated from a human umbilical vein endothelial cDNA library using a monoclonal antibody which reacts specifically to human endothelial cells and monocytes. This cDNA clone consists of 1469 nucleotides with an open reading frame of 1191 nucleotides. HDJ-2 shares significant homology with Escherichia coli heat shock protein DNAJ, as well as the yeast homologues Sec63, YDJ1, SCJ1 and SIS1. This homology suggests HDJ-2 may be involved in protein folding and/or transport.

摘要

利用一种可与人内皮细胞和单核细胞特异性反应的单克隆抗体，从人脐静脉内皮cDNA文库中分离出细菌DNAJ热休克蛋白的人同源物HDJ-2。该cDNA克隆由1469个核苷酸组成，开放阅读框为1191个核苷酸。HDJ-2与大肠杆菌热休克蛋白DNAJ以及酵母同源物Sec63、YDJ1、SCJ1和SIS1具有显著同源性。这种同源性表明HDJ-2可能参与蛋白质折叠和/或转运。