Svoboda M, Izakovicova Holla L, Sefr R, Vrtkova I, Kocakova I, Tichy B, Dvorak J
Oncobios Research Group, CZ 612 00 Brno, Czech Republic.
Int J Oncol. 2008 Sep;33(3):541-7.
There is increasing evidence that some microRNAs change their levels in reaction to xenobiotic challenge. The aim of this study was to test the possible involvement of micro-RNAs in response to standard anticancer treatment. Tumor biopsies from 35 patients with rectal cancer before therapy and parallel tumor biopsies from 31 patients two weeks after starting preoperative capecitabine chemoradiotherapy were taken. The expression levels of single miRNA species were measured using TaqMan Micro-RNA assays after reverse transcription from isolated total RNAs. Many micro-RNAs (miR10a, miR21, miR145, miR212, miR339, miR361) responded to chemoradiotherapy in individual tumor samples, but there was profound intertumoral variability. However, other two micro-RNAs miR125b, miR137 showed a significant increase in median expression levels after starting therapy in most samples. Moreover, our results for the first time show that higher induced levels of miR125b and miR137 are associated with worse response to the therapy.
越来越多的证据表明，一些微小RNA会因外源性物质的刺激而改变其水平。本研究的目的是测试微小RNA在对标准抗癌治疗反应中的可能作用。采集了35例直肠癌患者治疗前的肿瘤活检样本，以及31例患者在开始术前卡培他滨放化疗两周后的平行肿瘤活检样本。从分离的总RNA逆转录后，使用TaqMan微小RNA检测法测量单个微小RNA种类的表达水平。许多微小RNA（miR10a、miR21、miR145、miR212、miR339、miR361）在个别肿瘤样本中对放化疗有反应，但肿瘤间存在很大差异。然而，另外两个微小RNA miR125b、miR137在大多数样本开始治疗后，其表达水平的中位数显著增加。此外，我们的结果首次表明，miR125b和miR137的较高诱导水平与治疗反应较差有关。
