Ohno Yoshio, Izumi Miki, Yoshioka Kunihiko, Ohori Makoto, Yonou Hiroyuki, Tachibana Masaaki
Department of Urology, Tokyo Medical University, 6-7-1 Nishishinjuku, Shinjuku-ku, Tokyo, Japan.
Oncol Rep. 2008 Sep;20(3):511-6.
Tenascin-C is an extracellular matrix protein that plays an important role in cell proliferation, migration and tumor invasion in various types of cancer. However, few reports exist on tenascin-C expression in renal cell carcinoma (RCC). This study aimed to assess the prognostic significance of tenascin-C in clear cell RCC. Using immunohistochemistry, 137 formalin-fixed, paraffin-embedded tissue sections obtained from patients with clear cell RCC were examined for tenascin-C expression. Tenascin-C expression was observed in 55 (40.1%) of the 137 clear cell RCC sections. Tumor cells displayed membranous and/or cytoplasmic staining for tenascin-C. Tenascin-C expression was more prominent near the pseudocapsule of the tumor and around the tumor vessels. Tenascin-C expression was significantly associated with a higher stage (P=0.0065) and higher nuclear grade (P=0.0001). However, there was no correlation between the tenascin-C expression and venous involvement. The cancer-specific survival rate in patients with a tenascin-C-positive primary tumor was significantly lower than that in those with a tenascin-C-negative primary tumor in univariate analysis (P=0.0017). However, tenascin-C expression did not exhibit a significant value for cancer-related death in the Cox regression analysis. In patients with stage 1-3 disease, the 5-year metastasis-free rate in patients with the tenascin-C-positive primary tumor was significantly lower than that in those with the tenascin-C-negative primary tumor (67.8 vs. 88.5%, respectively; P=0.0038). The Cox regression analysis showed that tenascin-C expression is a significant predictor of metastasis (P=0.0345). The tenascin-C expression was strongly related to the stage, nuclear grade and 5-year metastasis-free rate. Therefore, tenascin-C expression may be a possible marker for the metastatic potential of clear cell RCC.
腱生蛋白-C是一种细胞外基质蛋白，在各类癌症的细胞增殖、迁移及肿瘤侵袭过程中发挥着重要作用。然而，关于腱生蛋白-C在肾细胞癌（RCC）中的表达情况，相关报道较少。本研究旨在评估腱生蛋白-C在透明细胞肾细胞癌中的预后意义。采用免疫组织化学方法，对137例取自透明细胞肾细胞癌患者的福尔马林固定、石蜡包埋组织切片进行腱生蛋白-C表达检测。在137例透明细胞肾细胞癌切片中，有55例（40.1%）观察到腱生蛋白-C表达。肿瘤细胞呈现出腱生蛋白-C的膜性和/或细胞质染色。腱生蛋白-C的表达在肿瘤假包膜附近及肿瘤血管周围更为显著。腱生蛋白-C表达与更高的分期（P=0.0065）及更高的核分级（P=0.0001）显著相关。然而，腱生蛋白-C表达与静脉侵犯之间并无相关性。在单因素分析中，原发性肿瘤腱生蛋白-C阳性患者的癌症特异性生存率显著低于腱生蛋白-C阴性患者（P=0.0017）。然而，在Cox回归分析中，腱生蛋白-C表达对癌症相关死亡并无显著意义。在1-3期疾病患者中，原发性肿瘤腱生蛋白-C阳性患者的5年无转移率显著低于腱生蛋白-C阴性患者（分别为67.8%和88.5%；P=0.0038）。Cox回归分析表明，腱生蛋白-C表达是转移的显著预测指标（P=0.0345）。腱生蛋白-C表达与分期、核分级及5年无转移率密切相关。因此，腱生蛋白-C表达可能是透明细胞肾细胞癌转移潜能的一个潜在标志物。
