Vigne E, Marmonier A, Fuchs M
Unité Mixte de Recherche Santé de la Vigne et Qualité du Vin, Institut National de la Recherche Agronomique and Université Louis Pasteur, 68021 Colmar, France.
Arch Virol. 2008;153(9):1771-6. doi: 10.1007/s00705-008-0182-y. Epub 2008 Aug 10.
Sequence alignments and SISCAN analyses inferred multiple interspecies recombination events within RNA2 of strains GHu of Grapevine fanleaf virus (GFLV) and Ta of Arabis mosaic virus (ArMV), two closely related subgroup A nepoviruses in the family Comoviridae. Interspecies recombination events were identified in the 5' untranslated region, the putative homing protein and movement protein genes but not in the coat protein gene and 3' untranslated region. These findings suggest a dynamic relationship between GFLV and ArMV, and a differential selection pressure on RNA2-encoded proteins with constraints in terms of function and co-adaptation that limit interspecies recombination to certain gene segments.
序列比对和SISCAN分析推断出葡萄扇叶病毒（GFLV）的GHu株和南芥菜花叶病毒（ArMV）的Ta株的RNA2内存在多个种间重组事件，这两种病毒是豆科病毒科中密切相关的A亚组线虫传多面体病毒。在5'非翻译区、推定的归巢蛋白和运动蛋白基因中鉴定到种间重组事件，但在外壳蛋白基因和3'非翻译区未鉴定到。这些发现表明GFLV和ArMV之间存在动态关系，并且对RNA2编码的蛋白质存在差异选择压力，在功能和共适应方面存在限制，从而将种间重组限制在某些基因片段。
