Vigne Emmanuelle, Marmonier Aurélie, Komar Véronique, Lemaire Olivier, Fuchs Marc
Institut National de la Recherche Agronomique and Université de Strasbourg, Unité Mixte de Recherche Santé de la Vigne et Qualité du Vin 1131, 68021 Colmar, France.
Virus Res. 2009 Sep;144(1-2):154-62. doi: 10.1016/j.virusres.2009.04.018. Epub 2009 May 4.
Recombination was assessed in a vineyard site in which grapevines cross-protected with mild strains GHu of Grapevine fanleaf virus (GFLV) or Ta of Arabis mosaic virus (ArMV) were superinfected with GFLV field isolates following transmission by the nematode vector Xiphinema index. The genetic structure and variability within RNA2 of isolates from grapevines co-infected with GFLV field isolates and either GFLV-GHu or ArMV-Ta were characterized to identify intra- and interspecies recombinants. Sequence analysis and phylogenetic relationships inferred intraspecies recombination among GFLV field isolates but not between field isolates and GFLV-GHu. SISCAN analysis confirmed a mosaic structure for two GFLV field isolates for which recombination sites were located in the movement protein and coat protein genes. One of the recombinants was found in eight grapevines that were in close spatial proximity within the vineyard site, suggesting its transmission by X. index. No interspecies recombination was detected between GFLV field isolates and ArMV-Ta. Altogether, our findings suggest that mild protective strains GFLV-GHu and ArMV-Ta did not assist the emergence of viable recombinants to detectable level during a 12-year cross-protection trial. To our knowledge, this is the first extensive characterization of the genetic structure and variability of virus isolates in cross-protected plants.
在一个葡萄园场地评估了重组情况，在该场地中，用葡萄扇叶病毒（GFLV）的温和毒株GHu或阿拉伯花叶病毒（ArMV）的Ta进行交叉保护的葡萄藤，在被线虫传毒介体索引剑线虫传播后，又被GFLV田间分离株超感染。对与GFLV田间分离株以及GFLV-GHu或ArMV-Ta共同感染的葡萄藤分离株的RNA2内的遗传结构和变异性进行了表征，以鉴定种内和种间重组体。序列分析和系统发育关系推断出GFLV田间分离株之间存在种内重组，但田间分离株与GFLV-GHu之间不存在种内重组。SISCAN分析证实了两个GFLV田间分离株的镶嵌结构，其重组位点位于运动蛋白和外壳蛋白基因中。其中一个重组体在葡萄园场地内空间距离较近的八株葡萄藤中被发现，表明它是由索引剑线虫传播的。在GFLV田间分离株与ArMV-Ta之间未检测到种间重组。总之，我们的研究结果表明，在一项为期12年的交叉保护试验中，温和保护株GFLV-GHu和ArMV-Ta并未促使有活力的重组体出现到可检测水平。据我们所知，这是首次对交叉保护植物中病毒分离株的遗传结构和变异性进行广泛表征。
