Illouz Severine, Alexandre Eliane, Pattenden Clare, Mark Louise, Bachellier Philippe, Webb M'Balu, Berry David, Dennison Ashley, Richert Lysiane
Department of Hepatobiliary and Pancreatic Surgery, Leicester General Hospital, Leicester, UK.
Phytother Res. 2008 Dec;22(12):1688-91. doi: 10.1002/ptr.2545.
Curcumin (CUR) is a major component of a dietary spice derived from the roots of Curcuma longa. It has strong antioxidant activities and hepatoprotective properties. Primary human hepatocytes are clinically used in transplantation or in bioartificial liver devices for the treatment of patients with liver failure. Fresh and cryopreserved hepatocytes are also used in vitro for the study of drugs in pharmacotoxicology. We aimed to assess whether CUR could improve human liver cell viability and prevent oxidative damage responsible for large cell loss during cell preparation. Our study showed beneficial effects of CUR (25 microM) on freshly isolated human hepatocytes, increasing significantly metabolic activity of viable attached cells when seeded with CUR for 24 h. However CUR added during the cell isolation process did not have any significant impact on cell isolation outcomes or on cryopreservation outcomes. Conversely, CUR added during the thawing of frozen cells had a negative effect on the cell attachment capacity of hepatocytes that were cryopreserved in the presence or absence of CUR. In conclusion, although having positive effects on viability and challenge of oxidative stress on cultured human hepatocytes, CUR had no beneficial effect on cell isolation or cryopreservation outcomes.
姜黄素（CUR）是从姜黄根中提取的一种膳食香料的主要成分。它具有强大的抗氧化活性和肝脏保护特性。原代人肝细胞在临床上用于移植或生物人工肝装置中，以治疗肝功能衰竭患者。新鲜和冷冻保存的肝细胞也在体外用于药物毒理学研究。我们旨在评估姜黄素是否能提高人肝细胞活力，并预防细胞制备过程中导致大量细胞损失的氧化损伤。我们的研究表明，姜黄素（25微摩尔）对新鲜分离的人肝细胞具有有益作用，当与姜黄素一起接种24小时时，可显著提高贴壁存活细胞的代谢活性。然而，在细胞分离过程中添加姜黄素对细胞分离结果或冷冻保存结果没有任何显著影响。相反，在冷冻细胞解冻过程中添加姜黄素对在有或没有姜黄素存在的情况下冷冻保存的肝细胞的细胞贴壁能力有负面影响。总之，尽管姜黄素对培养的人肝细胞的活力和氧化应激挑战有积极影响，但对细胞分离或冷冻保存结果没有有益作用。
