Troyo Adriana, Fuller Douglas O, Calderón-Arguedas Olger, Beier John C
Global Public Health Program, Department of Epidemiology and Public Health, University of Miami, 12500 SW 152 St. Building A, Miami, FL 33177, USA.
J Vector Ecol. 2008 Jun;33(1):1-7. doi: 10.3376/1081-1710(2008)33[1:agsmfs]2.0.co;2.
Entomological surveys in urban areas are often biased by selecting houses or locations with known high vector densities. A sampling strategy was developed for Puntarenas, Costa Rica, using high-resolution satellite imagery. Grids from the Advanced Spaceborne Thermal Emission and Reflection Radiometer and a QuickBird classified land cover map were used to determine the optimal final grid area for surveys. A random sample (10% of cells) was selected, and sample suitability was assessed by comparing the mean percentage of tree cover between sample and total cells. Sample cells were used to obtain entomological data from 581 locations: 26.3% of all locations positive for mosquito larvae were not households, they contained 29.5% of mosquito-positive habitats and 16% of Aedes aegypti pupae collected. Entomological indices for Ae. aegypti (pupae per person, Breteau index, container index, location index) were slightly lower when only household data were analyzed. High-resolution satellite imagery and geographical information systems appear useful for evaluating urban sites and randomly selecting locations for accurate entomological surveys.
城市地区的昆虫学调查往往因选择已知病媒密度高的房屋或地点而存在偏差。利用高分辨率卫星图像为哥斯达黎加的蓬塔雷纳斯制定了一种抽样策略。来自先进星载热发射和反射辐射计的网格以及一张快鸟分类土地覆盖图被用于确定调查的最佳最终网格区域。选取了一个随机样本（占单元格的10%），并通过比较样本单元格和总单元格之间树木覆盖的平均百分比来评估样本的适宜性。样本单元格被用于从581个地点获取昆虫学数据：所有蚊子幼虫呈阳性的地点中有26.3%不是家庭住所，这些地点包含29.5%的蚊子阳性栖息地以及所采集的埃及伊蚊蛹的16%。仅分析家庭数据时，埃及伊蚊的昆虫学指标（每人蛹数、布雷托指数、容器指数、地点指数）略低。高分辨率卫星图像和地理信息系统似乎有助于评估城市地点并随机选择地点进行准确的昆虫学调查。
