单纯疱疹病毒在保存的鼠角膜中的存活情况。

The survival of herpes simplex virus in preserved murine corneas.

作者信息

Robert Pierre-Yves, Ducher Virginie, Pleyer Uwe, Denis François, Adenis Jean-Paul, Ranger-Rogez Sylvie

机构信息

Department of Ophthalmology, EA 3175, CHU Dupuytren, Limoges, France.

出版信息

Ophthalmic Res. 2008 Oct;40(6):329-32. doi: 10.1159/000151245. Epub 2008 Aug 12.

DOI:10.1159/000151245

PMID:18698148

Abstract

BACKGROUND/AIMS: This study investigates the survival of HSV in infected mouse corneas, in the conditions of normal human eye bank preservation.

METHODS

Hundred seventy-two BALB/C mice infected with herpes simplex virus 1 (HSV-1) (KOS) were randomly assigned to either: no preservation in group 1 (n = 62), 31 degrees C preservation for 3 weeks in group 2 (n = 70) or 4 degrees C preservation for 8 days in group 3 (n = 40). The presence of HSV-1 was thereafter detected by viral culture and PCR.

RESULTS

In groups 1, 2 and 3, HSV-1 was detected by culture in 22 (35.5%), 1 (1.4%) and 0 (0.0%) of the corneas, and by PCR in 27 (43.7%), 3 (4.2%) and 7 (17.5%) of the corneas respectively. When compared to group 1, HSV was detected significantly less often in groups 2 (p < 0.0001) and 3 (p < 0.0001).

CONCLUSIONS

HSV-1 DNA undergoes a degradation during corneal preservation.

摘要

背景/目的：本研究在人眼库正常保存条件下，调查单纯疱疹病毒（HSV）在感染小鼠角膜中的存活情况。

方法

172只感染1型单纯疱疹病毒（HSV-1）（KOS株）的BALB/C小鼠被随机分为三组：第1组不进行保存（n = 62），第2组在31℃保存3周（n = 70），第3组在4℃保存8天（n = 40）。之后通过病毒培养和聚合酶链反应（PCR）检测HSV-1的存在情况。

结果

在第1、2和3组中，通过病毒培养分别在22只（35.5%）、1只（1.4%）和0只（0.0%）角膜中检测到HSV-1，通过PCR分别在27只（43.7%）、3只（4.2%）和7只（17.5%）角膜中检测到HSV-1。与第1组相比，第2组（p < 0.0001）和第3组（p < 0.0001）检测到HSV的频率显著降低。