Plagnol Vincent, Uz Elif, Wallace Chris, Stevens Helen, Clayton David, Ozcelik Tayfun, Todd John A
JDRF/WT Diabetes and Inflammation Laboratory, University of Cambridge, Cambridge, United Kingdom.
PLoS One. 2008 Aug 13;3(8):e2966. doi: 10.1371/journal.pone.0002966.
Lymphoblastoid cell lines (LCL) are being actively and extensively used to examine the expression of specific genes and genome-wide expression profiles, including allele specific expression assays. However, it has recently been shown that approximately 10% of human genes exhibit random patterns of monoallelic expression within single clones of LCLs. Consequently allelic imbalance studies could be significantly compromised if bulk populations of donor cells are clonal, or near clonal. Here, using X chromosome inactivation as a readout, we confirm and quantify widespread near monoclonality in two independent sets of cell lines. Consequently, we recommend where possible the use of bulk, non cell line, ex vivo cells for allele specific expression assays.
淋巴母细胞系（LCL）正被积极且广泛地用于检测特定基因的表达以及全基因组表达谱，包括等位基因特异性表达分析。然而，最近有研究表明，在LCL的单个克隆中，约10%的人类基因呈现出单等位基因表达的随机模式。因此，如果供体细胞的大量群体是克隆性的或近乎克隆性的，等位基因失衡研究可能会受到显著影响。在这里，我们以X染色体失活作为读数，确认并量化了两组独立细胞系中广泛存在的近乎单克隆性。因此，我们建议在可能的情况下，使用大量的、非细胞系的离体细胞进行等位基因特异性表达分析。
