Hayashida Osamu, Uchiyama Masaki
Institute for Materials Chemistry and Engineering, Kyushu University, Moto-oka 744, Fukuoka 819-0395, Japan.
Org Biomol Chem. 2008 Sep 7;6(17):3166-70. doi: 10.1039/b804431g. Epub 2008 Jul 8.
Rotaxane-type receptors, which were composed of anionic cyclophane-based resorcinarene tetramers as the wheel and a 2,6-disubstituted naphthalene derivative having two fluorophore moieties, such as fluorescein and rhodamine residues, as the axle ( and , respectively), were prepared. Rotaxane-type receptors and bound histone, a small basic protein component of eukaryotic chromatins, with binding constants of 2.3 x 10(6) and 9.0 x 10(5) M(-1), respectively. The rotaxane-type receptors showed fluorescence sensing ability with remarkable histone selectivity. Moreover, fluorescence resonance energy transfer (FRET) between the fluorescein residues of and the rhodamine residues of took place in the presence of histone, which was a useful method for the detection of histone.
制备了轮烷型受体，其由基于阴离子环番的间苯二酚杯芳烃四聚体作为轮以及具有两个荧光团部分（如荧光素和罗丹明残基）的2,6-二取代萘衍生物作为轴（分别为和）。轮烷型受体与组蛋白（真核染色质的一种小碱性蛋白质成分）结合，结合常数分别为2.3×10⁶和9.0×10⁵ M⁻¹。轮烷型受体表现出具有显著组蛋白选择性的荧光传感能力。此外，在组蛋白存在的情况下，和的荧光素残基与的罗丹明残基之间发生了荧光共振能量转移（FRET），这是一种检测组蛋白的有用方法。
