Calatzis Andreas, Peetz Dirk, Haas Sylvia, Spannagl Michael, Rudin Karin, Wilmer Marianne
Department of Haemostasis and Transfusion Medicine, Munich University Clinic, Munich, Germany.
Am J Clin Pathol. 2008 Sep;130(3):446-54. doi: 10.1309/Q0G21Y26UR0UHQ1A.
The prothrombinase-induced clotting time assay (PiCT, Pentapharm, Basel, Switzerland) is a clotting assay sensitive to factor Xa and factor IIa inhibitors. It is based on the addition of factor Xa and snake venom RVV-V (Russell viper venom factor V activator) specifically activating factor V and phospholipids to platelet-poor plasma. Following an incubation time, the mixture is recalcified and the clotting time is determined. An almost linear dose-response and high sensitivity of the assay for unfractionated heparin (UFH), low-molecular-weight heparins (LMWHs), r-hirudin, and argatroban was found. Fondaparinux showed a nonlinear dose-response. By using ex vivo samples, the following Pearson correlation coefficients were found: r=0.85 between amidolytic anti-Xa and PiCT for 120 LMWH and 24 control samples; r=0.86 between amidolytic anti-Xa activity and PiCT for 68 UFH and 24 control samples; and r=0.94 between ECT and PiCT for 38 hirudin samples. Thus, PiCT is a promising assay for the monitoring of anticoagulants inhibiting factor Xa and/or factor IIa.
凝血酶原酶诱导凝血时间测定法（PiCT，Pentapharm公司，瑞士巴塞尔）是一种对Xa因子和IIa因子抑制剂敏感的凝血测定法。它基于向乏血小板血浆中添加Xa因子和特异性激活V因子的蛇毒RVV-V（罗素蝰蛇毒因子V激活剂）以及磷脂。经过一段孵育时间后，向混合物中重新加入钙离子并测定凝血时间。该测定法对普通肝素（UFH）、低分子肝素（LMWHs）、重组水蛭素和阿加曲班呈现出几乎线性的剂量反应和高灵敏度。磺达肝癸钠呈现出非线性剂量反应。通过使用体外样本，得到了以下皮尔逊相关系数：120份LMWH和24份对照样本的酰胺水解抗Xa活性与PiCT之间r = 0.85；68份UFH和24份对照样本的酰胺水解抗Xa活性与PiCT之间r = 0.86；38份水蛭素样本的ECT与PiCT之间r = 0.94。因此，PiCT是一种用于监测抑制Xa因子和/或IIa因子的抗凝剂的有前景的测定法。
