Lei Xia, Wang En Tao, Chen Wen Feng, Sui Xin Hua, Chen Wen Xin
Key Laboratory of Agro-Microbial Resource and Application, Ministry of Agriculture/College of Biological Sciences, China Agricultural University, 100193 Beijing, China.
Arch Microbiol. 2008 Dec;190(6):657-71. doi: 10.1007/s00203-008-0418-y. Epub 2008 Aug 15.
In the present study, a total of 154 bacterial strains isolated from nodules of eighteen Vicia species mainly grown in the temperate Chinese provinces were characterized by ARDRA, ITS PCR-RFLP, BOX-PCR, sequencing of 16S rDNA, nodC, nifH, atpD and glnII, and nodulation tests. The results demonstrated that most of the R. leguminosarum strains were effective microsymbionts of the wild Vicia species, while genomic species related to Rhizobium gallicum, Mesorhizobium huakuii, Ensifer meliloti and Bradyrhizobium spp. were symbiotic bacteria occasionally nodulating with Vicia species. In addition, fourteen strains related to Agrobacterium, Phyllobacterium, Ensifer, Shinella and R. tropici, as well as 22 strains of R. leguminosarum might be nodule endophytes without symbiotic genes. Diverse symbiotic gene lineages were found among the test strains and a strong association was found among the symbiotic gene types and genomic species, indicating the absence of lateral gene transfer. These results greatly enlarged the rhizobial spectrum of Vicia species.
在本研究中，从主要生长在中国温带省份的18种野豌豆属植物根瘤中分离出的总共154株细菌菌株，通过ARDRA、ITS PCR-RFLP、BOX-PCR、16S rDNA、nodC、nifH、atpD和glnII测序以及结瘤试验进行了表征。结果表明，大多数豌豆根瘤菌菌株是野豌豆属野生植物的有效微共生体，而与法国根瘤菌、华癸中生根瘤菌、苜蓿中华根瘤菌和慢生根瘤菌属相关的基因组种是偶尔与野豌豆属植物结瘤的共生细菌。此外，14株与土壤杆菌属、叶杆菌属、中华根瘤菌属、新鞘氨醇菌属和热带根瘤菌相关的菌株，以及22株豌豆根瘤菌可能是没有共生基因的根瘤内生菌。在测试菌株中发现了不同的共生基因谱系，并且在共生基因类型和基因组种之间发现了很强的关联，表明不存在横向基因转移。这些结果极大地扩大了野豌豆属植物的根瘤菌谱。
