Yamada A, Imanishi J, Nakajima E, Nakajima K, Nakajima S
Department of Microbiology, Kyoto Prefectural University of Medicine.
Microbiol Immunol. 1991;35(3):259-65. doi: 10.1111/j.1348-0421.1991.tb01555.x.
An assay protocol based on exploiting the polymerase chain reaction (PCR) for the direct detection of influenza virus in throat swab is described. By use of the mixture of H1 and H3 primers, it was possible to determine the subtype of the influenza A viruses simultaneously. No visible band was detected after PCR of influenza B or A (H2N2) viruses with a pair of H1 or H3 primers. The dilution experiment showed that the influenza viruses, as few as 1.3-6 plaque-forming units, were sufficient for detecting the HA gene by PCR. All throat swab samples from which influenza viruses had been isolated by conventional method were also positively detected by PCR method.
本文描述了一种基于利用聚合酶链反应（PCR）直接检测咽喉拭子中流感病毒的检测方案。通过使用H1和H3引物混合物，能够同时确定甲型流感病毒的亚型。用一对H1或H3引物对乙型流感病毒或甲型（H2N2）流感病毒进行PCR后，未检测到可见条带。稀释实验表明，低至1.3 - 6个蚀斑形成单位的流感病毒就足以通过PCR检测HA基因。所有通过传统方法分离出流感病毒的咽喉拭子样本，用PCR方法也均检测为阳性。
