Roy S, Patil D, Dahake R, Mukherjee S, Athlekar S V, Deshmukh R A, Chowdhary A
Department of Virology, Haffkine Institute, Mumbai 400 012, India.
Indian J Med Microbiol. 2012 Apr-Jun;30(2):155-8. doi: 10.4103/0255-0857.96670.
Influenza has a major impact on public heath, annually affecting 15-20% of the global population. Information on the activity of influenza virus in Mumbai is limited. The present study was carried out to determine the prevalence of influenza viruses causing acute respiratory infections in children by molecular methods.
To study the prevalence of influenza viruses among the paediatric population in Mumbai by real-time reverse-transcriptase polymerase chain reaction (rRT-PCR).
From July 2007 to July 2009, 100 respiratory samples (nasal and throat swabs) were collected from paediatric patients with acute respiratory symptoms. attending out patients department, and admitted to the paediatric wards of B. J. Wadia Hospital for Children, Mumbai. The samples were collected and processed as per World Health Organization (WHO) guidelines. Viral RNA was extracted and one-step rRT-PCR was performed to detect influenza type A (H1 and H3) and influenza type B virus.
Out of 100 samples processed by rRT-PCR, a total of 11 samples (11%) were positive for influenza virus. The typing for influenza A subtypes showed 1% (1) positivity for H1 and 5% (5) positivity for H3 subtypes and 5% (5) samples tested positive for influenza type B virus.
It was observed that both influenza type A and B viruses were prevalent in Mumbai during the study period. Such surveillance data are important in the early detection of any antigenic variants that may be helpful in global influenza vaccine preparation and for any pandemic preparedness activity.
流感对公众健康有重大影响，每年影响全球15% - 20%的人口。孟买流感病毒活动的信息有限。本研究旨在通过分子方法确定引起儿童急性呼吸道感染的流感病毒的流行情况。
通过实时逆转录聚合酶链反应（rRT-PCR）研究孟买儿科人群中流感病毒的流行情况。
2007年7月至2009年7月，从孟买BJ瓦迪亚儿童医院门诊及儿科病房收治的有急性呼吸道症状的儿科患者中采集了100份呼吸道样本（鼻拭子和咽拭子）。样本按照世界卫生组织（WHO）指南进行采集和处理。提取病毒RNA，并进行一步rRT-PCR以检测甲型流感（H1和H3）和乙型流感病毒。
在通过rRT-PCR处理的100份样本中，共有11份样本（11%）流感病毒呈阳性。甲型流感亚型分型显示H1亚型阳性率为1%（1份），H3亚型阳性率为5%（5份），乙型流感病毒检测阳性的样本为5%（5份）。
研究期间观察到甲型和乙型流感病毒在孟买均有流行。此类监测数据对于早期发现任何可能有助于全球流感疫苗制备及任何大流行防范活动的抗原变异体非常重要。
