Amiel D, Gelberman R, Harwood F, Siegel D
Division of Orthopaedics and Rehabilitation, University of California San Diego, La Jolla.
Matrix. 1991 Jun;11(3):184-9. doi: 10.1016/s0934-8832(11)80157-5.
The medial and lateral forepaw flexor tendons of 20-adult mongrel dogs (n = 40) were transected and repaired with a modified Kessler suture. Post-operatively the dogs were subjected either to immobilization or early controlled passive digital motion. Sacrifices were at 3, 7, 10 and 17 days. The tendons of the contralateral limbs were left intact and used as controls. Urea-heparin-extracted fibronectin was quantitated by competitive ELISA in the tendons and sheaths at the four post-injury/repair time periods. In both groups (controlled passive motion and immobilization), fibronectin concentrations were higher in the injured tissues than in control tissues. However, peak fibronectin concentration (7-days post-injury/repair) was approximately twice as high in the controlled passive motion tissues as in the immobilized tissues. It was concluded, therefore, that, relative to early controlled passive motion, early immobilization depresses the accumulation of tissue fibronectin during the early stages of healing following injury.
对20只成年杂种犬（n = 40）的前爪内侧和外侧屈肌腱进行横断，并采用改良Kessler缝合进行修复。术后，将犬只分为固定组或早期控制性被动指动组。分别在术后3天、7天、10天和17天处死犬只。对侧肢体的肌腱保持完整，用作对照。采用竞争性酶联免疫吸附测定法（ELISA）对损伤/修复后四个时间段的肌腱和腱鞘中尿素-肝素提取的纤连蛋白进行定量分析。在两组（控制性被动运动组和固定组）中，损伤组织中的纤连蛋白浓度均高于对照组织。然而，控制性被动运动组组织中纤连蛋白的峰值浓度（损伤/修复后7天）约为固定组组织的两倍。因此得出结论，相对于早期控制性被动运动，早期固定会抑制损伤后愈合早期组织纤连蛋白的积累。
