Schmidt J, Winker H, Schmidt I, Ekkernkamp A, Merk H
Unfallchirurgie, HELIOS Klinikum Berlin-Buch.
Z Orthop Unfall. 2008 Jul-Aug;146(4):490-7. doi: 10.1055/s-2008-1038766.
The purpose of this study was to verify the possibility of monitoring the callus maturation biochemically.
In an experimental study on sheep, animals with bone fractures with a length 5 cm each were divided into group A (with concomitant damage of the soft tissue) and group B (without soft tissue damage). During callus traction and maturation, biochemical parameters (osteocalcin, AlP, NTx, calcium, phosphate) were measured. Animals of each group (A+B) were euthanised at fixed points in time (according to protocol) and the callus was prepared histologically.
Total AlP and NTx values were initially reduced during the callus traction and maturation, but increased significantly at the time of Docking+1 transportation time. At the same time, the histological evaluation showed a definite increase of the bone stabilisation, which approximately resembles the structure of a healthy bone as reference.
With the combination of the AlP and NTx values already determined in the daily clinical routine the monitoring of callus maturation in animal studies becomes possible.
本研究的目的是验证通过生化方法监测骨痂成熟的可能性。
在一项针对绵羊的实验研究中，将每处骨折长度为5厘米的动物分为A组（伴有软组织损伤）和B组（无软组织损伤）。在骨痂牵引和成熟过程中，测量生化参数（骨钙素、碱性磷酸酶、NTx、钙、磷）。每组（A+B）动物在固定时间点（根据方案）实施安乐死，并对骨痂进行组织学制备。
在骨痂牵引和成熟过程中，总碱性磷酸酶和NTx值最初降低，但在对接+1运输时间时显著升高。同时，组织学评估显示骨稳定性明显增加，大致类似于作为参照的健康骨骼结构。
结合日常临床常规中已测定的碱性磷酸酶和NTx值，在动物研究中监测骨痂成熟成为可能。
