Nakasato Fumika, Shimoyama Tadashi, Yoshimura Tetsuro, Mikami Tatsuya, Munakata Akihiro, Fukuda Shinsaku
First Department of Internal Medicine, Hirosaki University School of Medicine, Hirosaki 036-8562, Japan.
Hepatogastroenterology. 2008 May-Jun;55(84):1122-5.
BACKGROUND/AIMS: H. pylori uses the sialic acid-binding adhesin (SabA) to recognize Lewis X (LeX) antigen of gastric epithelial cells. SabA is associated with nonopsonic activation of human neutrophils. The aims of this study were to examine the association of bacterial sabA status to the presence of anti-LeX antibody in host and the grade of gastritis.
44 H. pylori strains cultured from gastric biopsies were examined by PCR for presence of 23SrRNA, cagA, and sabA. Serum samples were obtained from all the patients to measure the level of anti-LeX antibody. Histological grade of gastritis was graded according to the updated Sydney System.
23SrRNA gene and the cagA gene were seen in all the samples while 21 strains were sabA positive. The mean titer of anti-LeX antibody was 0.09 and 0.18 in patients infected with sabA-positive and -negative strain, respectively (NS). The grade of inflammatory infiltration was not significantly different between groups in both the corpus and the antral mucosa.
Possession of the sabA gene by infected H. pylori strain might not associate with the presence of anti-LeX antibody in the host. Possession of sabA gene by infected H. pylori might not associate with severity of gastric mucosal inflammation.
背景/目的：幽门螺杆菌利用唾液酸结合黏附素（SabA）识别胃上皮细胞的Lewis X（LeX）抗原。SabA与人类中性粒细胞的非调理激活有关。本研究的目的是检测细菌sabA状态与宿主中抗LeX抗体的存在以及胃炎分级之间的关联。
对从胃活检组织中培养的44株幽门螺杆菌菌株进行PCR检测，以确定是否存在23SrRNA、cagA和sabA。从所有患者中采集血清样本，以测量抗LeX抗体水平。根据更新后的悉尼系统对胃炎的组织学分级。
所有样本中均检测到23SrRNA基因和cagA基因，其中21株sabA呈阳性。感染sabA阳性和阴性菌株的患者中，抗LeX抗体的平均滴度分别为0.09和0.18（无显著性差异）。胃体和胃窦黏膜组间的炎症浸润分级无显著差异。
感染的幽门螺杆菌菌株携带sabA基因可能与宿主中抗LeX抗体的存在无关。感染的幽门螺杆菌携带sabA基因可能与胃黏膜炎症的严重程度无关。
