Low Rate of Genotype among Iranian Clinical Isolates.

INTRODUCTION

The Blood Group Antigen-Binding Adhesion (), Outer Inflammatory Protein () and Sialic Acid-Binding Adhesin () as outer membrane proteins involved in adherence to gastric mucosa have been suggested to have a role in the pathogenesis.

AIM

To investigate the frequency of isolates , and genes in Iranian dyspeptic patients.

MATERIALS AND METHODS

DNAs were extracted from -positive cultures taken from 100 different dyspeptic patients. Genotyping was performed by Polymerase Chain Reaction (PCR), using the specific primers for , and genes. Chi square test was used to investigate association between variables, p<0.05 was considered statistically significant.

RESULTS

All (100%) isolates possessed and genotypes, whereas was detected in 22% of isolates. There was no significant relationship between presence of genes with clinical outcome. The combined genotype +/ +/ - was correlated with gastritis. The rate of genotype in our isolates was lower than other Iranian reports.

CONCLUSION

Frequency of genotype among isolates from Southwest of Iran is considerably less than other regions of Iran. Due to heterogeneity of strains in different geographic regions, further work will be needed to understand the role of these virulence genes in pathogenesis and their possible association with disease outcome.