Huang Sheng-Fu, Li Yu-Hong, Ren Yi-jin, Cao Zheng-Guo, Long Xing
Department of Orthodontics, School of Stomatology, Wuhan University, Wuhan, China.
Eur J Oral Sci. 2008 Aug;116(4):319-23. doi: 10.1111/j.1600-0722.2008.00552.x.
Matrix metalloproteinase-1 (MMP-1) 1G/2G (-1,607) polymorphisms have been identified and shown to influence the transcription of the MMP-1 gene. In order to compare the expression of MMP-1 with different MMP-1 gene promoter alleles after force loading, human periodontal ligament (PDL) cells were cultured and genotyped into three alleles by polymerase chain reaction and restriction endonuclease cleavage. The three genotypes of PDL cells were centrifuged and the expression of MMP-1 mRNA and protein were determined by reverse transcription-polymerase chain reaction and enzyme-linked immunosorbent assay. The results showed that centrifugal force upregulated the expression of both MMP-1 mRNA and protein in all three genotypes of PDL cells. The induction of MMP-1 by force was significantly greater in cells with a 2G/2G genotype or a 1G/2G genotype than in cells homozygous for the 1G allele. The MMP-1 mRNA and protein levels were significantly higher for cells with the 2G allele than for cells with the 1G/2G allele or the 1G allele. These results suggest that a single nucleotide polymorphism in the -1,607 bp MMP-1 promoter region might be associated with the difference observed in the endogenous expression of MMP-1 in PDL cells under mechanical force.
基质金属蛋白酶-1（MMP-1）1G/2G（-1,607）多态性已被鉴定，并显示会影响MMP-1基因的转录。为了比较受力加载后不同MMP-1基因启动子等位基因的MMP-1表达情况，培养了人牙周膜（PDL）细胞，并通过聚合酶链反应和限制性内切酶切割将其基因分型为三种等位基因。对三种基因型的PDL细胞进行离心处理，并通过逆转录-聚合酶链反应和酶联免疫吸附测定法测定MMP-1 mRNA和蛋白的表达。结果表明，离心力上调了所有三种基因型PDL细胞中MMP-1 mRNA和蛋白的表达。与1G等位基因纯合的细胞相比，2G/2G基因型或1G/2G基因型的细胞中，力对MMP-1的诱导作用明显更大。具有2G等位基因的细胞中MMP-1 mRNA和蛋白水平显著高于具有1G/2G等位基因或1G等位基因的细胞。这些结果表明，MMP-1启动子区域-1,607 bp处的单核苷酸多态性可能与机械力作用下PDL细胞中MMP-1内源性表达的差异有关。
