Deval Christiane, Talvas Jérémie, Chaveroux Cédric, Maurin Anne-Catherine, Mordier Sylvie, Cherasse Yoan, Parry Laurent, Carraro Valérie, Jousse Céline, Bruhat Alain, Fafournoux Pierre
Unité de Nutrition Humaine, UMR 1019, INRA de Theix, Saint Genès Champanelle, France.
Biochimie. 2008 Nov-Dec;90(11-12):1716-21. doi: 10.1016/j.biochi.2008.07.004. Epub 2008 Jul 25.
There is a growing body of evidence that suggests that amino acids play an important role in controlling gene expression, but the cell specificity of the amino-acid-mediated regulation of gene expression in mammals remains unknown. Using a model of muscle cells (C2C12) at two stages of differentiation, i.e. myoblasts and myotubes, we employed transcriptional profiling to show that amino-acid deficiency does not regulate the same set of gene in differentiated and non-differentiated cells. Furthermore, in myotubes, the GCN2 pathway is not activated by amino-acid starvation due to an amino-acid supply from intracellular proteolysis associated with a low GCN2 expression.
越来越多的证据表明，氨基酸在控制基因表达中发挥着重要作用，但氨基酸介导的哺乳动物基因表达调控的细胞特异性仍不清楚。利用处于两个分化阶段的肌肉细胞（C2C12）模型，即成肌细胞和肌管，我们采用转录谱分析表明，氨基酸缺乏在分化细胞和未分化细胞中调控的基因集不同。此外，在肌管中，由于与低GCN2表达相关的细胞内蛋白质水解提供的氨基酸供应，GCN2途径不会因氨基酸饥饿而被激活。
